Protein Info for GFF423 in Pseudomonas sp. DMC3

Annotation: Tol-Pal system protein TolQ

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 317 signal peptide" amino acids 1 to 37 (37 residues), see Phobius details transmembrane" amino acids 100 to 122 (23 residues), see Phobius details amino acids 209 to 231 (23 residues), see Phobius details amino acids 252 to 275 (24 residues), see Phobius details TIGR02797: tonB-system energizer ExbB" amino acids 90 to 299 (210 residues), 346.3 bits, see alignment E=3.6e-108 PF01618: MotA_ExbB" amino acids 168 to 282 (115 residues), 111 bits, see alignment E=1.7e-36

Best Hits

Swiss-Prot: 78% identical to EXBB_PSEPU: Biopolymer transport protein ExbB (exbB) from Pseudomonas putida

KEGG orthology group: K03561, biopolymer transport protein ExbB (inferred from 94% identity to pfo:Pfl01_5555)

Predicted SEED Role

"MotA/TolQ/ExbB proton channel family protein" in subsystem Ton and Tol transport systems

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (317 amino acids)

>GFF423 Tol-Pal system protein TolQ (Pseudomonas sp. DMC3)
MTRNQTPASPTTRPRAWSAVAALLLSLMLAPTAAFADAQAPATPAATEQSAPAAAPAAPA
ATDPVQAVDAADAPEVLEADNTLGMAHDLSPWGMYQNADIIVKIVMIGLAIASIITWTIW
IAKGFELMGAKRRLRGEIAALKKATTLKEASATAGKEGTLANLLVHDALEEMRLSVNSRE
KEGIKERVSFRLERLVAACGRNMSSGTGVLATIGSTAPFVGLFGTVWGIMNSFIGIAKTQ
TTNLAVVAPGIAEALLATALGLVAAIPAVVIYNVFARSIAGYKAQVSDASAEVLLLVSRD
LDHQPERSSQPHMVKVG