Protein Info for GFF4229 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Exopolyphosphatase (EC 3.6.1.11)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 494 TIGR03706: exopolyphosphatase" amino acids 7 to 301 (295 residues), 310.2 bits, see alignment E=7.4e-97 PF02541: Ppx-GppA" amino acids 23 to 302 (280 residues), 240.9 bits, see alignment E=2e-75 PF21447: Ppx-GppA_III" amino acids 310 to 478 (169 residues), 119.1 bits, see alignment E=2e-38

Best Hits

Swiss-Prot: 40% identical to GPPA_VIBC3: Guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase (gppA) from Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 / O395)

KEGG orthology group: K01524, exopolyphosphatase / guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase [EC: 3.6.1.11 3.6.1.40] (inferred from 63% identity to vei:Veis_1294)

Predicted SEED Role

"Exopolyphosphatase (EC 3.6.1.11)" in subsystem Phosphate metabolism (EC 3.6.1.11)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.6.1.11 or 3.6.1.40

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (494 amino acids)

>GFF4229 Exopolyphosphatase (EC 3.6.1.11) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MQNGTLLAAIDLGSNSFRLEIGRYHSGHIERIEYLKETVRQGTGLDEEKNLSQAAMERGW
ECLARFAERLHGFKKQQVRAVATQTLREAKNRDEFLRNAQAILGFSIDVVSGHEEARLIY
QGVSRLLPHSDEKRLVVDIGGRSTEMILGQGYTAHAMESYRLGSVAWSTRYFPRGQFTAA
AFKTAEVAAKAVIDEALDKFPTPEWTVAYGSSGTVGAVADMLAANGWASGVVTRTGLDWL
TERMIKAGSADQLRLEGLKDDRRPVIGGGVSVLRAIFDLFGIEQMLPAQGALRHGALYDL
IDRDTDGGDVRERTVRWLAQRFSTDGLQAQRVSEVSTALFSQIAALDAQNERYSQKLAWA
ARLHEIGTHISHERSHHHGAYILDNVDAPGFSLPELHRMSQLVMGQRGKLRKLEEALMDE
LFAKQLMALRLAVLLCHARQMPEYQSVKLSYKPRAFKVSTNPGWAKRYPQSAWLLGEEVV
AWQKSAWKFTADIR