Protein Info for PS417_21660 in Pseudomonas simiae WCS417

Annotation: alanyl-tRNA synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01411: tRNA-synt_2c
  • amino acids 5 to 551 (547 residues), 755.3 bits, E=5.7e-231
• TIGR00344: alanine--tRNA ligase
  • amino acids 5 to 851 (847 residues), 1072.9 bits, E=0
• PF07973: tRNA_SAD
  • amino acids 649 to 693 (45 residues), 50.4 bits, 2.7e-17
• PF02272: DHHA1
  • amino acids 731 to 862 (132 residues), 96.9 bits, E=2.2e-31

Best Hits

Swiss-Prot: 65% identical to SYA_HAHCH: Alanine--tRNA ligase (alaS) from Hahella chejuensis (strain KCTC 2396)

KEGG orthology group: K01872, alanyl-tRNA synthetase [EC: 6.1.1.7] (inferred from 82% identity to avn:Avin_34460)

MetaCyc: 65% identical to alanine--tRNA ligase/DNA-binding transcriptional repressor (Escherichia coli K-12 substr. MG1655)
Alanine--tRNA ligase. [EC: 6.1.1.7]; 6.1.1.7 [EC: 6.1.1.7]; 6.1.1.7 [EC: 6.1.1.7]; RXN-19726 [EC: 6.1.1.7, 3.1.1.96]; 3.1.1.- [EC: 6.1.1.7, 3.1.1.96]

Predicted SEED Role

"Alanyl-tRNA synthetase (EC 6.1.1.7)" (EC 6.1.1.7)

MetaCyc Pathways

• tRNA charging (20/21 steps found)
• superpathway of chorismate metabolism (44/59 steps found)
• shinorine biosynthesis (3/8 steps found)
• enterobactin biosynthesis (5/11 steps found)
• vanchrobactin biosynthesis (2/8 steps found)
• bacillibactin biosynthesis (4/12 steps found)
• ergotamine biosynthesis (4/12 steps found)
• (2S,3E)-2-amino-4-methoxy-but-3-enoate biosynthesis (2/10 steps found)
• superpathway of ergotamine biosynthesis (5/18 steps found)
• sulfazecin biosynthesis (1/16 steps found)
• phosalacine biosynthesis (4/25 steps found)
• phosphinothricin tripeptide biosynthesis (4/25 steps found)
• corallopyronin A biosynthesis (2/30 steps found)
• colibactin biosynthesis (6/38 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Aminoacyl-tRNA biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.1.96 or 6.1.1.7

Sequence Analysis Tools

See A0A1N7U5D5 at UniProt or InterPro

Protein Sequence (872 amino acids)

>PS417_21660 alanyl-tRNA synthetase (Pseudomonas simiae WCS417)
MKSAEIREAFLRFFEEQGHTRVASSSLIPGNDPTLLFTNAGMNQFKDCFLGQEKRAYTRA
VSSQKCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKQDAINFAWTFLTGVLKLP
KEKLWVTVYASDDEAYDIWTKEVGVPAERMVRIGDNKGAPYASDNFWTMGDTGPCGPCTE
IFYDHGADIWGGPPGSPEEDGDRYIEIWNNVFMQFNRTADGVLHPLPAPSVDTGMGLERI
SAVMQHVHSNYEIDLFQSLLAAAAKAIGCTNEDQASLKVVADHIRSCGFLIADGVLPSNE
GRGYVLRRIIRRACRHGNKLGANGSFFYQIVAALVAEMGEAFPELKQNQAHIERVLKAEE
EQFAKTLEQGLKILEQDLANLQGSVVPGDVVFKLYDTYGFPMDLTGDIARERNLTLDEEG
FEREMEAQRVRARSASSFGMDYNSLVKVDVATEFTGYKATRGAAKVVALYKEGQSVDVLN
EGDDGVVVLDQTPFYAESGGQIGDCGFLEASSGRFDVRDTTKTGGAFLHHGVLASGSLTV
GSPVEAQVDADVRHATSLNHSATHLLHAALRQVLGEHVQQKGSLVDSQRLRFDFSHFEAI
KPEQIKALEDIVNAEIRKNTAVETEETDIETAKAKGAMALFGEKYGDSVRVLSMGGDFSV
ELCGGIHANRTGDIALLKIVSEGGVASGVRRIEAVTGAAALAYLNAAEEQLKEAASLVKG
SRDNLIDKLSAVLERNRALEKQLEQLQAKAASAAGDDLSASAVDVKDVKVLAARLDGQDG
KALLALVDQLKNKLGRAVILLGGVHEDKVVLVAGVTKDLTGQLKAGDLMKQAAAAVGGKG
GGRPDMAQGGGVDAGALDAALALTVPFVEAGI