Protein Info for GFF4226 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Polyphosphate kinase (EC 2.7.4.1)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 692 TIGR03705: polyphosphate kinase 1" amino acids 12 to 667 (656 residues), 862.2 bits, see alignment E=1.4e-263 PF13089: PP_kinase_N" amino acids 13 to 113 (101 residues), 108 bits, see alignment E=7.7e-35 PF02503: PP_kinase" amino acids 123 to 305 (183 residues), 199.4 bits, see alignment E=1.1e-62 PF17941: PP_kinase_C_1" amino acids 333 to 496 (164 residues), 229.2 bits, see alignment E=5.6e-72 PF13090: PP_kinase_C" amino acids 505 to 665 (161 residues), 175.6 bits, see alignment E=1.6e-55

Best Hits

Swiss-Prot: 47% identical to PPK1_ACIB3: Polyphosphate kinase (ppk) from Acinetobacter baumannii (strain AB307-0294)

KEGG orthology group: K00937, polyphosphate kinase [EC: 2.7.4.1] (inferred from 73% identity to pol:Bpro_2246)

Predicted SEED Role

"Polyphosphate kinase (EC 2.7.4.1)" in subsystem High affinity phosphate transporter and control of PHO regulon or Phosphate metabolism or Purine conversions (EC 2.7.4.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.4.1

Use Curated BLAST to search for 2.7.4.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (692 amino acids)

>GFF4226 Polyphosphate kinase (EC 2.7.4.1) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MSGNTLNQKHLLLDRDHSILAFNERVMDWARRPDVPLLERLRYLSIVSSNLDEFFEVRVA
PHLTAARANEQRGLYTSQSYEELSDKVHSLVAQQYAIYNDELLPLLEKKGIKLISHGERN
QRQRRWVKEFFTKEVQPLLMPVGLDPAHPFPQVANKSLNFIVRLTGKDAFGRENEIAIVK
VPRILPRFFRMPPVKGSRQTHFVSISSLIRSHLNDLFPGRQVTEFSQFRVTRHSDLAVDE
EEVKNLRTALRKGLQQRHYGQALRLEVSAGCSVHLSNFLLEQFALPERALYRVPGPVNLV
RLNQLIDQVDAPALRFETYNAGWPAQLTPGQSYFERLRQGDVLIHQPFESFDAVLGFLRE
AVEDPDVLAIKQTIYRTGAKSELMNLLREAVRRGKEVTAVVELKARFDEEANINYAERLE
SVGAQVVYGVVGLKTHAKMLLVTRREASGLRRYAHLSTGNYNPGTARLYTDLSYLTADDA
LTADMEQIFLHLASQNRLPKLNKALIAPFHLHKAMLDKVEAAGAAAKAGQEARIVLKMNA
LTDEPMARALAVASQQGVKIDLIIRGACILPAQVPGFTDNVQVRSVIGRLLEHSRVFYFR
TGEVEELWLSSADWMNRNMLRRIELAWPVTDSALRRRIIDECLQAYLHDTKDAWLLRPDG
SYTPAGAVGNGRSRTMLHSAQASLMALHGSKG