Protein Info for GFF4204 in Variovorax sp. SCN45

Annotation: Oxidoreductase, short-chain dehydrogenase/reductase family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00106: adh_short
  • amino acids 9 to 200 (192 residues), 167.4 bits, E=5.6e-53
• PF08659: KR
  • amino acids 11 to 157 (147 residues), 35.5 bits, E=1.9e-12
• PF13561: adh_short_C2
  • amino acids 14 to 247 (234 residues), 197.8 bits, E=4.2e-62

Best Hits

Swiss-Prot: 34% identical to KDUD_DICD3: 2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase (kduD) from Dickeya dadantii (strain 3937)

KEGG orthology group: K00059, 3-oxoacyl-[acyl-carrier protein] reductase [EC: 1.1.1.100] (inferred from 96% identity to vap:Vapar_5496)

MetaCyc: 34% identical to KduD (Dickeya dadantii 3937)
2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase. [EC: 1.1.1.127]

Predicted SEED Role

"3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100)" in subsystem Fatty Acid Biosynthesis FASII or mycolic acid synthesis (EC 1.1.1.100)

MetaCyc Pathways

• superpathway of fatty acids biosynthesis (E. coli) (48/53 steps found)
• superpathway of fatty acid biosynthesis II (plant) (38/43 steps found)
• palmitate biosynthesis II (type II fatty acid synthase) (29/31 steps found)
• biotin biosynthesis I (14/15 steps found)
• oleate biosynthesis IV (anaerobic) (13/14 steps found)
• superpathway of unsaturated fatty acids biosynthesis (E. coli) (17/20 steps found)
• superpathway of fatty acid biosynthesis I (E. coli) (14/16 steps found)
• 8-amino-7-oxononanoate biosynthesis I (10/11 steps found)
• palmitoleate biosynthesis I (from (5Z)-dodec-5-enoate) (8/9 steps found)
• fatty acid elongation -- saturated (5/5 steps found)
• gondoate biosynthesis (anaerobic) (4/4 steps found)
• (5Z)-dodecenoate biosynthesis I (5/6 steps found)
• stearate biosynthesis II (bacteria and plants) (5/6 steps found)
• anteiso-branched-chain fatty acid biosynthesis (25/34 steps found)
• even iso-branched-chain fatty acid biosynthesis (25/34 steps found)
• odd iso-branched-chain fatty acid biosynthesis (25/34 steps found)
• 8-amino-7-oxononanoate biosynthesis IV (4/5 steps found)
• cis-vaccenate biosynthesis (4/5 steps found)
• octanoyl-[acyl-carrier protein] biosynthesis (mitochondria, yeast) (9/12 steps found)
• palmitate biosynthesis III (21/29 steps found)
• (5Z)-dodecenoate biosynthesis II (4/6 steps found)
• stearate biosynthesis IV (4/6 steps found)
• 4-deoxy-L-threo-hex-4-enopyranuronate degradation (3/5 steps found)
• tetradecanoate biosynthesis (mitochondria) (17/25 steps found)
• 3,6-anhydro-α-L-galactopyranose degradation (4/7 steps found)
• petroselinate biosynthesis (2/6 steps found)
• 2-allylmalonyl-CoA biosynthesis (2/8 steps found)
• streptorubin B biosynthesis (20/34 steps found)
• superpathway of microbial D-galacturonate and D-glucuronate degradation (17/31 steps found)
• mycolate biosynthesis (26/205 steps found)
• superpathway of mycolate biosynthesis (27/239 steps found)

KEGG Metabolic Maps

• Biosynthesis of unsaturated fatty acids
• Fatty acid biosynthesis
• Pentose and glucuronate interconversions

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.100

Use Curated BLAST to search for 1.1.1.100 or 1.1.1.127

Protein Sequence (252 amino acids)

>GFF4204 Oxidoreductase, short-chain dehydrogenase/reductase family (Variovorax sp. SCN45)
MNMSLAGRAAFISGAGHGIGRATARAMARLGATVGVNDLKPEFVEEAVAEIRALGGQAIG
LPCDVSTREGMREAVALFAREAGRLDVMVNNAAWVRYQPLAEITPEVMDRMVEIGFKSVI
WGIQAAAEAMDPQRGGSIVNVASIAAFKSPMRSVVYSGIKAGVLGITRASAADLGGRNIR
VNAVAPGAIPTEGTAKHRNAELDAKRIGRTPLGRLGTVEDIADAVCFLAGDASRFFNADV
LRLDGGASETSL