Protein Info for GFF4204 in Pseudomonas sp. DMC3

Annotation: Dimethyl-sulfide monooxygenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 452 TIGR03860: FMN-dependent oxidoreductase, nitrilotriacetate monooxygenase family" amino acids 10 to 433 (424 residues), 555.4 bits, see alignment E=3.9e-171 PF00296: Bac_luciferase" amino acids 32 to 391 (360 residues), 176 bits, see alignment E=6.6e-56

Best Hits

KEGG orthology group: None (inferred from 94% identity to pfo:Pfl01_0238)

Predicted SEED Role

"Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (452 amino acids)

>GFF4204 Dimethyl-sulfide monooxygenase (Pseudomonas sp. DMC3)
MSAAKKKILLNAFNMNCIGHINHGLWTHPRDTSTRYNTIEYWTELAQLLERGLFDGLFIA
DIVGVYDVYQNSVDVTLKESIQLPVNDPLLLVSAMAAVTRNLGFGLTANLTYEPPYLFAR
RMSTLDHLSRGRVGWNIVTGYLDSAAKAMGLREQVEHDRRYDQAEEYLQVLYKLWEGSWE
NGAVLNDREQRIYANPDKVHKVEHRGEFYQVEGYHLCEPSPQRTPVLFQAGSSDRGLLFA
GRHAECVFISGQNKASTKVQVDKVRASAVEAGRNPQDIKVFMGLNVIVGATEEAAWAKHA
EYLSYASAEAGVAHFSASTGIDFAQYEIDEPIQYVKNNAIQSATKNLQNNDWTRRKLLDQ
HALGGRYITVVGSPEQVADELESWIAETGLDGFNLTRIVTPESYVDFIELVIPELQRRGS
YKTAYDSGTLREKLFHGEAQLPEQHTGSSYRR