Protein Info for GFF4175 in Pseudomonas sp. DMC3

Annotation: Phosphoenolpyruvate carboxykinase (ATP)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 99% identical to PCKA_PSEPF: Phosphoenolpyruvate carboxykinase (ATP) (pckA) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K01610, phosphoenolpyruvate carboxykinase (ATP) [EC: 4.1.1.49] (inferred from 99% identity to pfo:Pfl01_0268)

Predicted SEED Role

"Phosphoenolpyruvate carboxykinase [ATP] (EC 4.1.1.49)" in subsystem Pyruvate metabolism I: anaplerotic reactions, PEP or Serine-glyoxylate cycle (EC 4.1.1.49)

MetaCyc Pathways

gluconeogenesis I (11/13 steps found)
superpathway of glyoxylate cycle and fatty acid degradation (11/14 steps found)
C4 photosynthetic carbon assimilation cycle, PEPCK type (9/14 steps found)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.1.49

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (513 amino acids)

>GFF4175 Phosphoenolpyruvate carboxykinase (ATP) (Pseudomonas sp. DMC3)
MTQANNAVYTDLSVDDLVKEALNRGEGELADTGALVVRTGHRTGRSPVDRFIVEEPSTQA
AIAWGPINRKFPADKFDALWARVEAFNNAQEHFVSHVHVGAAEDHYLAVKMTTQTAWQNL
FGRCLFINPAQYNPAGREEWQVLNVANFECVPERDGTNSDGCVIINFAQKKVLIAGMRYA
GEMKKAMFSVQNFLLPAADVLPMHCAANIGEDGDVTLFFGLSGTGKTTLSADESRYLIGD
DEHGWGEGVVFNIEGGCYAKCIDLSEKNEPVIWKAIKHGAVLENVVIDDAKHADYADVSL
TQNSRAAYPLEHVAKRSEKNLGGEPNAVIFLTCDLTGVLPPVSILNEEQAAYHFLSGYTA
LVGSTEMGSGSGIKSTFSTCFGAPFFPRPAGEYAELLIKRIRGFGSKVYLVNTGWTGGGY
GVGQRFNIPTTRAVIAAIQSGALIGAATEHLDTINLDVPLAVPGVDTNLLNPRNTWADKA
AYDEAAKALAGLFIENFKKFEVSDAIKAAGPKL