Protein Info for GFF4164 in Xanthobacter sp. DMC5

Annotation: Bifunctional transcriptional activator/DNA repair enzyme Ada

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 309 PF12833: HTH_18" amino acids 56 to 132 (77 residues), 52.4 bits, see alignment E=7.9e-18 TIGR00589: methylated-DNA--[protein]-cysteine S-methyltransferase" amino acids 221 to 299 (79 residues), 104.7 bits, see alignment E=9.9e-35 PF01035: DNA_binding_1" amino acids 222 to 301 (80 residues), 111.4 bits, see alignment E=2.3e-36

Best Hits

KEGG orthology group: K10778, AraC family transcriptional regulator, regulatory protein of adaptative response / methylated-DNA-[protein]-cysteine methyltransferase [EC: 2.1.1.63] (inferred from 89% identity to xau:Xaut_2813)

Predicted SEED Role

"ADA regulatory protein / Methylated-DNA--protein-cysteine methyltransferase (EC 2.1.1.63)" in subsystem DNA repair, bacterial (EC 2.1.1.63)

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.63

Use Curated BLAST to search for 2.1.1.63

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (309 amino acids)

>GFF4164 Bifunctional transcriptional activator/DNA repair enzyme Ada (Xanthobacter sp. DMC5)
VLALDAPDLILSAAAPTLTATPAAQLDIAARDYDVVRRAVEYISCNVRRQPEVEEIAAAT
GVSARALTDLFRRWCGLTPKAFLQAVTLDRARRIVAECPNVLDAALELGLSGPGRLHDLF
VVHEAMSPGEWKTGGAGLVIRFGFHPSPFGQALAMSTPRGLCGLAFADLGEEAAALADMR
SRWPNATYLEDPAATAPLVAQIFDPASCRGDQPLRIVFIGTDFEIRVWETLLRIPMGRAT
TYSTIAEHVGRPKAARAVGAAIGKNPISFVVPCHRVIGKSGDLTGYHWGLTRKRAILGWE
AAKVEPKAE