Protein Info for PS417_21305 in Pseudomonas simiae WCS417

Annotation: electron transfer flavoprotein-ubiquinone oxidoreductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 554 PF01946: Thi4" amino acids 4 to 57 (54 residues), 27.4 bits, see alignment 5.7e-10 PF00890: FAD_binding_2" amino acids 9 to 52 (44 residues), 24.8 bits, see alignment 3.7e-09 PF13450: NAD_binding_8" amino acids 12 to 57 (46 residues), 28.7 bits, see alignment 4e-10 PF21162: ETFQO_UQ-bd" amino acids 213 to 309 (97 residues), 137.1 bits, see alignment E=7.5e-44 PF05187: ETF_QO" amino acids 450 to 551 (102 residues), 169.6 bits, see alignment E=5.3e-54

Best Hits

Swiss-Prot: 85% identical to ETFD_PSEAE: Electron transfer flavoprotein-ubiquinone oxidoreductase (PA2953) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K00311, electron-transferring-flavoprotein dehydrogenase [EC: 1.5.5.1] (inferred from 100% identity to pfs:PFLU4678)

Predicted SEED Role

"Electron transfer flavoprotein-ubiquinone oxidoreductase (EC 1.5.5.1)" in subsystem Acetyl-CoA fermentation to Butyrate or Anaerobic respiratory reductases (EC 1.5.5.1)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.5.5.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7U7N0 at UniProt or InterPro

Protein Sequence (554 amino acids)

>PS417_21305 electron transfer flavoprotein-ubiquinone oxidoreductase (Pseudomonas simiae WCS417)
MEREYMEFDVVIVGAGPAGLSAACRLKQKAAEAGKEISVCVVEKGSEVGAHILSGAVFEP
RALNELFPDWKELGAPLNTPVVRDDIYVLRSPEASTKVPDFFVPKTMHNEGNYIISLGNL
CRWLAQQAENLGVEVYPGFAAQEALFDENGVVRGIITGDLGVDREGNPKEGVYTPGMELR
GKYTLFAEGCRGHIGKQLIQRFNLDSDADAQHYGIGLKEIWEIDPAKHQPGLVVHTAGWP
LDIMSAENTGGSFLYHLENNQVVVGLIVDLSYSNTFLSPFDEFQRLKHHPVLAQYLEGGK
RISYGARAICKGGLNSLPKMVFKGGALIGCDLGTLNFAKIKGSHTAMKSGMLAADAVADR
LFAESEGGDELTAYVDSFKSSWLYEELFASRNFGPAMHKFGPIIGAGFNWFDQNILGGKM
PFTLHDTKPDYACLKLAKDSKKIDYPKPDGKLSFDKLSSVFISGTNHEEEQPCHLKLKDP
SIPIGTNLPLYDEPAQRYCPAGVYEVITKEDGEKRFQINAQNCVHCKTCDIKDPSQNITW
VTPEGAGGPTYPNM