Protein Info for GFF4123 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Type II/IV secretion system secretin RcpA/CpaC, associated with Flp pilus assembly

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 456 signal peptide" amino acids 1 to 50 (50 residues), see Phobius details PF13629: T2SS-T3SS_pil_N" amino acids 50 to 125 (76 residues), 53.5 bits, see alignment E=1.6e-18 PF00263: Secretin" amino acids 254 to 414 (161 residues), 159.1 bits, see alignment E=8.3e-51

Best Hits

KEGG orthology group: K02280, pilus assembly protein CpaC (inferred from 66% identity to vap:Vapar_4445)

Predicted SEED Role

"Type II/IV secretion system secretin RcpA/CpaC, associated with Flp pilus assembly" in subsystem Widespread colonization island

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (456 amino acids)

>GFF4123 Type II/IV secretion system secretin RcpA/CpaC, associated with Flp pilus assembly (Hydrogenophaga sp. GW460-11-11-14-LB1)
MFKPTPRPLPSRRAPRWPRLALLAGLLITAASGGLFAQPATDAPASASAARPLRLVAGTQ
QELVIDKGVERIAIANEQVATVMVTRQARGTPGARLIVTGKAPGSTSLLVWERGSDAATT
YTVEVQRAGAVLQGSYPSAAEHQQAREAALALQPDKAALADRSTLNVKSQTVQVEVKIVE
FNKRVLKQAGLNLFSTAPNKHGFSFGVFSPSSLQSYGFDRNASFTVEASSPLAQAFSLLL
GFGKANIGLNLGLLEGNGLARVLAEPTLVALSGQSANFLAGGEIPVPVAQGLGTTSIEYK
PFGIGLTVSPTVLSNQRIVLKVAPEASDLDYTNAVTLSGVAVPAITTRRADTTVELGDGE
SFVIGGLVSRTTTSSVDKVPMLGDLPVLGTVFKRQDYQQNERELVIVVTPHLVKPFAQGT
QVDAALPGRSEQRDPAFWRGFVVGNGADDALPGFSR