Protein Info for GFF4119 in Xanthobacter sp. DMC5

Annotation: 4,5:9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1 (10),2-diene-4-oate hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 320 signal peptide" amino acids 1 to 28 (28 residues), see Phobius details PF00561: Abhydrolase_1" amino acids 61 to 298 (238 residues), 127.5 bits, see alignment E=1.1e-40 PF12697: Abhydrolase_6" amino acids 62 to 305 (244 residues), 79.3 bits, see alignment E=1e-25 PF12146: Hydrolase_4" amino acids 62 to 298 (237 residues), 59.1 bits, see alignment E=6.4e-20

Best Hits

KEGG orthology group: None (inferred from 62% identity to bbt:BBta_5011)

Predicted SEED Role

"Lactone-specific esterase"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (320 amino acids)

>GFF4119 4,5:9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1 (10),2-diene-4-oate hydrolase (Xanthobacter sp. DMC5)
MSSRPTNTQIYLAAGVAATVAAVVANALIARKTERNNPPNGKFIEVDGVRLHYVERGIGQ
PLVLLHGNGVSIDDLESSGLLDLAANHHRVIAFDRPGFGHSSRPRATVWTAEAQAALFHK
ALLQLGVPRFLLFGHSWGACVAVRLAADYPQAVAGLVLASGYYYPGFRADALSMGAPAVP
GFGDVLRYTVSPLLGWLMWPKMLRDLFGPAAVPAKFGAFPRDMALRPSQLRASAAEAMLM
IPAAYGASEKYSDLRMPVAIVAGEGDRIVDIDDQSSRLHDDIPGSTFRRIKGGGHMVHQT
ATGEVMSAIDDVEREIATRM