Protein Info for GFF4104 in Variovorax sp. SCN45

Annotation: Cystathionine beta-lyase (EC 4.4.1.8)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 404 TIGR01324: cystathionine beta-lyase" amino acids 14 to 396 (383 residues), 408.2 bits, see alignment E=1.4e-126 PF01053: Cys_Met_Meta_PP" amino acids 14 to 394 (381 residues), 349.7 bits, see alignment E=8e-109

Best Hits

Swiss-Prot: 54% identical to METC_BORAV: Cystathionine beta-lyase (metC) from Bordetella avium

KEGG orthology group: K01760, cystathionine beta-lyase [EC: 4.4.1.8] (inferred from 90% identity to vap:Vapar_1884)

Predicted SEED Role

"Cystathionine beta-lyase (EC 4.4.1.8)" in subsystem Methionine Biosynthesis (EC 4.4.1.8)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.4.1.8

Use Curated BLAST to search for 4.4.1.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (404 amino acids)

>GFF4104 Cystathionine beta-lyase (EC 4.4.1.8) (Variovorax sp. SCN45)
MSQATRGKTLGAGTRLLHAGAPALRDGAGPVNVPVVRTSTVRFENTAAHADYHHRRTAGE
RVAAYGRHGLDTHRALEDAVTDLEGGHRAFLTPSGLSAITLVLLALLSPGDHALVADGVY
APLRRVDSTLLKRLGVQVEYFSPAHDDLASRIRPNTRLVYLESPSSLLFEVLDLPALTAV
ARDRGVTVATDNTWSGGWFYRPLALGANISIQAATKYISGHSDLMQGVVVVDSPALAAKL
STAYEALGLTIGADDAYLALRGLRTLPVRLAQHQRHATQVAEFLQQQPQVSRVFYPALPS
DPGHALWKRDFTGASGLVSFELRHATPAGANAFVDALALFGIGASWGGYESLALVAQPER
LREHRQWTGDAPVVRLHIGLEDPADLIADLSQAFAAAATAAAPR