Protein Info for GFF4103 in Variovorax sp. SCN45

Annotation: N-methylhydantoinase A (EC 3.5.2.14)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 718 PF05378: Hydant_A_N" amino acids 17 to 193 (177 residues), 179 bits, see alignment E=1.1e-56 PF01968: Hydantoinase_A" amino acids 214 to 509 (296 residues), 244 bits, see alignment E=3e-76 PF19278: Hydant_A_C" amino acids 555 to 690 (136 residues), 44.1 bits, see alignment E=2.3e-15

Best Hits

KEGG orthology group: K01473, N-methylhydantoinase A [EC: 3.5.2.14] (inferred from 54% identity to bbt:BBta_4925)

Predicted SEED Role

"N-methylhydantoinase A (EC 3.5.2.14)" in subsystem Hydantoin metabolism (EC 3.5.2.14)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.5.2.14

Use Curated BLAST to search for 3.5.2.14

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (718 amino acids)

>GFF4103 N-methylhydantoinase A (EC 3.5.2.14) (Variovorax sp. SCN45)
MSSNSNPSSATLDARVRVAVDVGGTFTDVVLLRGADKHTLKVLTTPTAPEQAVLGGVEEV
LQQANAGWADVDLLILGTTLATNALIERKGAPTALLTTHGFRDLVEIGLEDRFAQYDVFL
DKPAPLVPRHWRHGVVERVDAAGRVITPLDEAQVAALAGQLLADGIESVAVCFLHGYANP
AHERRVRELLREHAPSLWVSLASDVCSEIREYERLSTISANAYVQPQVAGYLRRLQEGAT
ARGLRAEPFLMTSGGAITTLQAGIDEPVRLVESGPAGGAVLARQVAEQIGASRALSFDMG
GTTAKICFIDDYQPQISRSFEFGRVHRHLKGSGLPIRIPVIEMVEIGAGGGSIARVNHLG
VVQVGPDSAGSSPGPAAYGLGGELPTVTDAHAALGTIDPARFAVGKVTLDPQRARDALLQ
GLTTQTGLDIESAAQAVVEIVTENMANAARVHASELGKVAEESTLIAFGGAAPLHAALLA
RKLGVARLVIPNSAGVGSALGFLWAPVAYQTVRSLAQRLDRIDHAAVDRLLAELTATADD
VVLRAAPGAALVRHRQAFMRYAGQGHEIPVELPEGTFDAAASKALHQRFEERYAALYGRS
LPHIAAEAVSWSVAVEAGGRAAPEPDEVVADTGRAEHAGTRRLYDAEAGQWTDVPVYERQ
ALDAGKWIEGPALIVEDETTTHVVAGFEARATLLGALVLDDKASVRHANAGINEEAEA