Protein Info for GFF4103 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: L-proline glycine betaine binding ABC transporter protein ProX (TC 3.A.1.12.1)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 340 signal peptide" amino acids 1 to 30 (30 residues), see Phobius details PF04069: OpuAC" amino acids 44 to 321 (278 residues), 164.5 bits, see alignment E=1.8e-52

Best Hits

Swiss-Prot: 66% identical to OUSX_DICD3: Glycine betaine-binding periplasmic protein OusX (ousX) from Dickeya dadantii (strain 3937)

KEGG orthology group: K02002, glycine betaine/proline transport system substrate-binding protein (inferred from 78% identity to aav:Aave_1495)

MetaCyc: 64% identical to glycine betaine ABC transporter periplasmic binding protein ProX (Escherichia coli K-12 substr. MG1655)
RXN-8638 [EC: 7.6.2.9]; 7.4.2.- [EC: 7.6.2.9]

Predicted SEED Role

"L-proline glycine betaine binding ABC transporter protein ProX (TC 3.A.1.12.1)" in subsystem Choline and Betaine Uptake and Betaine Biosynthesis (TC 3.A.1.12.1)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.6.2.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (340 amino acids)

>GFF4103 L-proline glycine betaine binding ABC transporter protein ProX (TC 3.A.1.12.1) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MTFPTPTPLWRTLLGGGLMALALTGPAQAQNLPGQGVAVQPLQSSIAEETFQTLLVSRAL
AKLGYDVKPIKEVEYPTAHVAIANGDATFMADHWDPLHADFYKNAGGDAKLYRQGAYSPG
AAQGYLIDRKTAEAHKITRVDQLKDPAIAKLFDHNGDGKADLTGCNPGWGCEAVIEHHLD
AYKLRDTVTHVQGSYAALIADTITRLKQGQPVLYYTWTPYWVSGVLRPGQDVVWLQVPFS
SLPGEQSKLDTQLPNGQNYGFVLNTQRIVANRQFTEQNPAAARLFEVMALPVADINAQNL
RMREGQNKPQDVERHTDAWIKAHQKTFDGWISQALAAAKR