Protein Info for GFF4099 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Fumarate hydratase class I, aerobic (EC 4.2.1.2)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF05681: Fumerase
  • amino acids 12 to 290 (279 residues), 333.1 bits, E=1.3e-103
• TIGR00722: hydrolyase, tartrate alpha subunit/fumarate domain protein, Fe-S type
  • amino acids 12 to 291 (280 residues), 217.2 bits, E=2.5e-68
• PF05683: Fumerase_C
  • amino acids 294 to 494 (201 residues), 279.3 bits, E=1.5e-87
• TIGR00723: hydrolyase, tartrate beta subunit/fumarate domain protein, Fe-S type
  • amino acids 332 to 491 (160 residues), 153.6 bits, E=4.8e-49

Best Hits

KEGG orthology group: K01676, fumarate hydratase, class I [EC: 4.2.1.2] (inferred from 91% identity to pol:Bpro_2046)

Predicted SEED Role

"Fumarate hydratase class I, aerobic (EC 4.2.1.2)" in subsystem Serine-glyoxylate cycle or TCA Cycle (EC 4.2.1.2)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (22/26 steps found)
• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (18/22 steps found)
• TCA cycle IV (2-oxoglutarate decarboxylase) (8/9 steps found)
• TCA cycle V (2-oxoglutarate synthase) (8/9 steps found)
• superpathway of glyoxylate bypass and TCA (10/12 steps found)
• partial TCA cycle (obligate autotrophs) (7/8 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (11/14 steps found)
• TCA cycle I (prokaryotic) (8/10 steps found)
• superpathway of anaerobic energy metabolism (invertebrates) (13/17 steps found)
• TCA cycle VIII (Chlamydia) (5/6 steps found)
• TCA cycle II (plants and fungi) (7/9 steps found)
• TCA cycle III (animals) (7/10 steps found)
• TCA cycle VI (Helicobacter) (6/9 steps found)
• TCA cycle VII (acetate-producers) (6/9 steps found)
• mixed acid fermentation (11/16 steps found)
• reductive TCA cycle I (7/11 steps found)
• incomplete reductive TCA cycle (4/7 steps found)
• pyruvate fermentation to propanoate I (4/7 steps found)
• anaerobic energy metabolism (invertebrates, mitochondrial) (6/10 steps found)
• methylaspartate cycle (12/19 steps found)
• (S)-lactate fermentation to propanoate, acetate and hydrogen (7/13 steps found)
• reductive TCA cycle II (6/12 steps found)
• L-glutamate degradation VIII (to propanoate) (4/11 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (21/56 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Citrate cycle (TCA cycle)
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.2

Use Curated BLAST to search for 4.2.1.2

Protein Sequence (517 amino acids)

>GFF4099 Fumarate hydratase class I, aerobic (EC 4.2.1.2) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MTTTIRQADLIESIAAALQYISYYHPADYIAHLARAYQREQSPAAKDAIAQILTNSKMSA
TGQRPICQDTGIVNVFLKVGMNVRWEGFTGGLEDAINEGVRRGYNHPDNTLRASVVADPQ
FARKNTKDNTPAVIVTELVPGDTVEVTVAAKGGGSENKSKMIMLNPGDSVVDWVLKTVPT
MGAGWCPPGMLGIGIGGTAEKAVLMAKESLMDDIDMYELQAKASKGEKLDQVEELRLELY
EKVNALGIGAQGLGGLTTVLDVKIKMYPTHAASKPVAMIPNCAATRHAHFVMDGSGPVYL
DAPSLDLWPNVNWVPDTQKSKRVDLNTLTKEEVASWKPGDTLLLNGKMLTGRDAAHKRIK
DMLAKGEKLPVDFTNRVIYYVGPVDPVRDEAVGPAGPTTATRMDGFTEMMLAETGLIAMI
GKSERGPVAIEAIKKHQSAYLMAVGGAAYLVSKAIKTAKVLGFADLGMEAIYEFDVVDMP
VTVAVDAGGTSAHITGPAEWQKRIATGEFKGIEVAAA