Protein Info for PS417_20930 in Pseudomonas simiae WCS417

Annotation: 50S rRNA methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 754 PF22020: RlmL_1st" amino acids 6 to 61 (56 residues), 57.7 bits, see alignment 2.3e-19 PF02926: THUMP" amino acids 70 to 156 (87 residues), 61.1 bits, see alignment E=2.7e-20 PF01170: UPF0020" amino acids 165 to 380 (216 residues), 142.9 bits, see alignment E=2.6e-45 PF10672: Methyltrans_SAM" amino acids 513 to 708 (196 residues), 69.8 bits, see alignment E=5.9e-23 PF03602: Cons_hypoth95" amino acids 588 to 676 (89 residues), 30.4 bits, see alignment E=7.7e-11

Best Hits

Swiss-Prot: 91% identical to RLMKL_PSEPF: Ribosomal RNA large subunit methyltransferase K/L (rlmL) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K12297, ribosomal RNA large subunit methyltransferase L [EC: 2.1.1.173] (inferred from 98% identity to pfs:PFLU4601)

Predicted SEED Role

"23S rRNA (guanine-N-2-) -methyltransferase rlmL EC 2.1.1.-)"

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.173

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7UQ47 at UniProt or InterPro

Protein Sequence (754 amino acids)

>PS417_20930 50S rRNA methyltransferase (Pseudomonas simiae WCS417)
MSDRFELFLTCPKGLEGLLIEEAVGLGLEEAREHTSAVRGMADMETAYRLCLWSRLANRV
LLVLKRFPMKDAEDLYHGVLDIEWADHMVPDGTLAVEFSGHGSGIDNTHFGALKVKDAIV
DKLRTPTGERPSIDKINPDLRIHLRLDRGEAILSLDLSGHSLHQRGYRLQQGAAPLKENL
AAAILIRAGWPRIAAEGGALSDPMCGVGTFLVEGAMIAADMAPNLNRELWGFTTWLGHVP
ALWKKLHAEAAERAAVGMNKPPLWIRGYEADPRLIQPARNNIERAGLSHWIKVYQGEVGT
FEPRPDQNQKGLVICNPPYGERLGDEASLLYLYQNLGERLRQACMGWEAAVFTGAPDLGK
RMGIRSHKQYSFWNGALPCKLLLIKVNPDQFVTGERRTPEQRQAEREQAAYDQAPAEPQE
RQYNKNGNPIKPAPAPVVEQARLSEGGQMFANRLQKNLKLLGKWAKREGVDCYRVYDADM
PEYSMAIDLYHDWVHVQEYAAPKSIDPEKASARMFDALAAIPQALNIDKSRVVVKRRERQ
SGTKQYERQSAQGKFTEVSEGGVKLLVNLTDYLDTGLFLDHRPMRLRIQKEAAGKRFLNL
YCYTATASVHAAKGGARSTTSVDLSKTYLDWARRNFSLNGFSDKNRLEQGDVMAWLETSR
DEFDLIFIDPPTFSNSKRMEGIFDVQRDHVQLLDLAMARLAPGGVLYFSNNFRKFALEDN
LSERYAVEEISDKTIDPDFARNAKIHRAWKITAR