Protein Info for GFF4083 in Variovorax sp. SCN45

Annotation: Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 811 transmembrane" amino acids 149 to 167 (19 residues), see Phobius details amino acids 178 to 197 (20 residues), see Phobius details amino acids 211 to 235 (25 residues), see Phobius details amino acids 246 to 266 (21 residues), see Phobius details amino acids 401 to 423 (23 residues), see Phobius details amino acids 429 to 451 (23 residues), see Phobius details amino acids 745 to 765 (21 residues), see Phobius details amino acids 771 to 792 (22 residues), see Phobius details PF04945: YHS" amino acids 35 to 79 (45 residues), 43.4 bits, see alignment 6.8e-15 PF19335: HMBD" amino acids 104 to 130 (27 residues), 51.3 bits, see alignment (E = 2.2e-17) TIGR01511: copper-translocating P-type ATPase" amino acids 192 to 795 (604 residues), 575.6 bits, see alignment E=2.5e-176 TIGR01525: heavy metal translocating P-type ATPase" amino acids 211 to 794 (584 residues), 604.6 bits, see alignment E=4.6e-185 TIGR01494: HAD ATPase, P-type, family IC" amino acids 255 to 764 (510 residues), 290.9 bits, see alignment E=3e-90 PF00122: E1-E2_ATPase" amino acids 284 to 465 (182 residues), 190.8 bits, see alignment E=4.1e-60 PF00702: Hydrolase" amino acids 482 to 699 (218 residues), 118 bits, see alignment E=1.8e-37 PF08282: Hydrolase_3" amino acids 673 to 730 (58 residues), 21.5 bits, see alignment 4.5e-08

Best Hits

KEGG orthology group: K01533, Cu2+-exporting ATPase [EC: 3.6.3.4] (inferred from 75% identity to pol:Bpro_1178)

Predicted SEED Role

"Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4)" (EC 3.6.3.3, EC 3.6.3.4, EC 3.6.3.5)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.3, 3.6.3.4, 3.6.3.5

Use Curated BLAST to search for 3.6.3.3 or 3.6.3.4 or 3.6.3.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (811 amino acids)

>GFF4083 Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4) (Variovorax sp. SCN45)
MSPTPSNLHEHHQGHHRAHPPASGEPSPETDATLKDPVCGMTVTTQSKHVLQHEGGSVYF
CSAGCKSKFEADPTKYAKRSAQAAAPLVLAPADSAQTNPVGTIYTCPMHPEIRQDHPGNC
PKCGMSLEPEMPTLEEGEDPELKDFKRRFFWTLPLTVVVTVLAMAGHRFQWFEMGMQSWI
ELVLTVPIVLWAGWPFFERAVQSVANRSPNMWTLIGLGTAAAFVYSVVATVAPGVFPASF
VSMGRVAVYFEAAAVIISLTLLGQILELKARSQTSAAIKSLLGLAPKTARRIGPDGAEED
VPIGHVHVGDKLRVRPGEKVPVDGVVIEGSSAVDESMLTGEPLPVTKRVGDKLIGATMNT
NGALVMQSEKVGSQTVLASIVQMVAQAQRSRAPMQRMADQVAGYFVMTVIAIAVLTFFAW
GFFGPQPSWVYGLINAVAVLIIACPCALGLATPMSIMVATGKAATQGVLFRDAAAIENFR
KVDALIVDKTGTLTEGRPQFERAVPAPGFTEDEVLRLAASLDQGSEHPLADAIVRAARER
NLVLDTPDGFESSSGIGVSGGVGGKKLALGNTALMEQLRVQVDDLKPRAEAMRAEGASVM
FLAVDGQPAGLLAVSDPIKATTMEALAALKASGMRVIMATGDGLTTARAVAAKLGIDEVH
GEVKPADKLALVDKLQREGRIVAMAGDGINDAPALAKADVGVAMGTGTDVAMNSAQVTLV
KGDLRGIAQARVISEQTIANMKQNLGFAFLYNALGVPLAAGVLFPFTGWLLSPMIAALAM
SLSSASVITNALRLRGAKSDVHASHVGERST