Protein Info for PS417_20885 in Pseudomonas simiae WCS417

Updated annotation (from data): Xanthine dehydrogenase (EC 1.17.1.4)
Rationale: Specifically important for utilizing Inosine. Automated validation from mutant phenotype: the predicted function (1.17.1.4) was linked to the condition via a SEED subsystem. This annotation was also checked manually.
Original annotation: FAD-binding molybdopterin dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02963: xanthine dehydrogenase, small subunit
  • amino acids 2 to 468 (467 residues), 697.9 bits, E=2.9e-214
• PF00111: Fer2
  • amino acids 17 to 76 (60 residues), 28.5 bits, E=2.4e-10
• PF01799: Fer2_2
  • amino acids 87 to 158 (72 residues), 97.7 bits, E=6.7e-32
• PF00941: FAD_binding_5
  • amino acids 199 to 362 (164 residues), 175.3 bits, E=2e-55
• PF03450: CO_deh_flav_C
  • amino acids 368 to 468 (101 residues), 123.4 bits, E=7.9e-40

Best Hits

KEGG orthology group: K13481, xanthine dehydrogenase small subunit [EC: 1.17.1.4] (inferred from 98% identity to pfs:PFLU4592)

Predicted SEED Role

"Xanthine dehydrogenase, iron-sulfur cluster and FAD-binding subunit A (1.17.1.4)" in subsystem Purine Utilization

MetaCyc Pathways

• purine nucleotides degradation II (aerobic) (11/11 steps found)
• adenosine nucleotides degradation II (5/5 steps found)
• purine nucleotides degradation I (plants) (10/12 steps found)
• adenosine nucleotides degradation I (7/8 steps found)
• guanosine nucleotides degradation II (4/4 steps found)
• guanosine nucleotides degradation III (4/4 steps found)
• inosine 5'-phosphate degradation (4/4 steps found)
• superpathway of purines degradation in plants (14/18 steps found)
• ureide biosynthesis (6/7 steps found)
• superpathway of guanosine nucleotides degradation (plants) (5/6 steps found)
• guanosine nucleotides degradation I (3/4 steps found)
• purine nucleobases degradation II (anaerobic) (16/24 steps found)
• caffeine degradation III (bacteria, via demethylation) (1/7 steps found)
• theophylline degradation (1/9 steps found)
• caffeine degradation IV (bacteria, via demethylation and oxidation) (1/10 steps found)

KEGG Metabolic Maps

• Purine metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.17.1.4

Use Curated BLAST to search for 1.17.1.4

Sequence Analysis Tools

See A0A1N7U079 at UniProt or InterPro

Protein Sequence (482 amino acids)

>PS417_20885 Xanthine dehydrogenase (EC 1.17.1.4) (Pseudomonas simiae WCS417)
MIQFLLNQELRSEHALDPNLTVLNYLREHLGKSGTKEGCASGDCGACTVVVGELHTDDTG
AEQIRYRSLNSCLTFVSSLHGKQLISVEDLKHQGQLHSVQKAMVECHGSQCGFCTPGFVM
SLFALQKNSDAPDNQKAHEALAGNLCRCTGYRPILAAAEQACCNKPQDQFDSRQAETIAR
LKAIAPTQTGELNSGDKRCLVPLTVADLADLYDAYPQARLLAGGTDLALEVTQFHRTLPV
MIYVGNIEEMKRIDDFDDRLEIGAAAALSDCYTALHHAYPDFGELLHRFASLQIRNQGTL
GGNIGNASPIGDSPPLLIALGAQIVLCKGETRRTLALEDYFIDYRVTARQDSEFIEKIIV
PKGHALFRAYKVSKRLDDDISAVCAAFNLKIDNGVIGEARVAFGGMAATPKRAKNCEAAL
VGATWNCATVEKACAALAEDFTPLSDFRASKEYRLLSAQNLLRKYFIELQTPHIETRVTA
YV