Protein Info for GFF4068 in Variovorax sp. SCN45

Annotation: Heavy metal sensor histidine kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 465 transmembrane" amino acids 39 to 63 (25 residues), see Phobius details amino acids 166 to 189 (24 residues), see Phobius details TIGR01386: heavy metal sensor kinase" amino acids 31 to 459 (429 residues), 338.6 bits, see alignment E=3.2e-105 PF00512: HisKA" amino acids 245 to 310 (66 residues), 51.3 bits, see alignment E=1e-17 PF02518: HATPase_c" amino acids 355 to 462 (108 residues), 73.9 bits, see alignment E=1.5e-24

Best Hits

Predicted SEED Role

"Heavy metal sensor histidine kinase" in subsystem Cobalt-zinc-cadmium resistance

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (465 amino acids)

>GFF4068 Heavy metal sensor histidine kinase (Variovorax sp. SCN45)
VSAAKTEAQELRPATETVAVPPQRRAQGTGSIQTALSRWFAAQALIGLSLVCLAIYAVTA
WSFQVKQASEFERQSELIKHLIQESRDDPGRDGLRHKLDDFFSTHAEISLVLRRDDEIVY
SSTPARTSGRWIWMPMQEQAMPSGDAAMQLQLGIDVREDAKILARLAWTLVGAVVLGSAV
ISLTGALLVRRGMQPLKKLVQQTAATGPEHPGRRIDPAPYASEITPWVAQFNAVLDRAEQ
AYQQLESFNADVAHELRTPLANLIGMVEVELARPRSIEELRDALLSALEEARRVSAIVID
MLFLSKADRGTVARRSAPVSLADQVRTVLDFHEATLEDAGLKVHVSGDADIGIDAGLVRR
ALSNLLSNASRYAAPGSTIAVVIDTQPDGVWVKVANRGEPVPADVLPNLFKRFFRAERSR
TDSSEHHGLGLAIVAAIARMHGGQTRATSRSGVTEISFSLGLPES