Protein Info for PS417_20760 in Pseudomonas simiae WCS417

Annotation: carbonate dehydratase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 816 transmembrane" amino acids 181 to 200 (20 residues), see Phobius details amino acids 212 to 234 (23 residues), see Phobius details amino acids 246 to 268 (23 residues), see Phobius details amino acids 274 to 292 (19 residues), see Phobius details amino acids 427 to 447 (21 residues), see Phobius details amino acids 453 to 478 (26 residues), see Phobius details amino acids 753 to 770 (18 residues), see Phobius details amino acids 776 to 796 (21 residues), see Phobius details PF12156: ATPase-cat_bd" amino acids 7 to 89 (83 residues), 98.2 bits, see alignment E=1.1e-31 PF00403: HMA" amino acids 96 to 156 (61 residues), 31.9 bits, see alignment 4.2e-11 TIGR01511: copper-translocating P-type ATPase" amino acids 231 to 796 (566 residues), 464.1 bits, see alignment E=1.5e-142 TIGR01525: heavy metal translocating P-type ATPase" amino acids 248 to 796 (549 residues), 510 bits, see alignment E=2.1e-156 TIGR01512: cadmium-translocating P-type ATPase" amino acids 275 to 797 (523 residues), 370.4 bits, see alignment E=2.9e-114 TIGR01494: HAD ATPase, P-type, family IC" amino acids 284 to 527 (244 residues), 115 bits, see alignment E=6.2e-37 amino acids 609 to 773 (165 residues), 110.7 bits, see alignment E=1.3e-35 PF00122: E1-E2_ATPase" amino acids 310 to 487 (178 residues), 168.9 bits, see alignment E=2.5e-53 PF00702: Hydrolase" amino acids 504 to 708 (205 residues), 102.1 bits, see alignment E=1.6e-32 PF12710: HAD" amino acids 625 to 704 (80 residues), 28.2 bits, see alignment E=7.3e-10 PF08282: Hydrolase_3" amino acids 679 to 739 (61 residues), 37.6 bits, see alignment 6.3e-13

Best Hits

KEGG orthology group: K01533, Cu2+-exporting ATPase [EC: 3.6.3.4] (inferred from 97% identity to pfs:PFLU4565)

Predicted SEED Role

"Type cbb3 cytochrome oxidase biogenesis protein CcoI; Copper-translocating P-type ATPase (EC 3.6.3.4)" (EC 3.6.3.4)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.4

Use Curated BLAST to search for 3.6.3.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7U4W3 at UniProt or InterPro

Protein Sequence (816 amino acids)

>PS417_20760 carbonate dehydratase (Pseudomonas simiae WCS417)
MTSPIPCYHCALPVPPGSRFTAVILGERRELCCPGCQAVAEAIVAGGLESYYQHRSEASA
NPEALPVQLVDELALYDRADVQKPFVRHEGELAEATLLMEGISCAACGWLIEKHLRSLPA
VAEARLNLSNHRLQVRWADGQLPLSQLLSELRHIGYAAHPYQPDRAAEQLASENRLALRQ
LGVAGLLWFQAMMATMATWPEFNIDLSPELHVILRWVAMFLTTPIVFYSCAPFFKGAMRD
LRTRHLTMDVSVSLAIGGAYLAGIWTAITGVGELYFDAVGMFALFLLAGRYLERRARERT
AAATAQLVNLLPASCLRLKADGQSERILLPELALGDRVLVHPGAVLPADGVILAGQSSID
ESLLTGEYLPQPRQAGDAVTAGTLNVEGALTVEVRALGHDTRLSAIVRLLERAQAEKPRL
AQIADRAAQWFLLCSLMAAALIGLLWWELDASRAFWIVLAMLVATCPCALSLATPTALTA
ATGTLHKLGLLLTRGHVLEGLNQIDTVIFDKTGTLTEGRLALRAIRPLGPLSTDLCLSLA
AALENRSEHPIARAFGRAPLAANEVISSPGLGLEGRVGERVLRIGQPGFVCELSGCPIPA
EPQVSGQWLLLGDREGALAWFVLDDRLRSDAPALLAACKARGWRTLLLSGDSSPMVASVA
AELGIDEAHGGLRPDDKLQVLQQLHKEGRKVLMLGDGVNDVPVLAAADISVAMGTATDLA
KTSADAVLLSNRLDALVQAFTLARRTRRVIIENLLWAGLYNGLMLPFAALGWITPIWAAV
GMSLSSLTVVLNALRLTRLPSAPATRAPSVTRPLPV