Protein Info for GFF4044 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: ATP-dependent protease La (EC 3.4.21.53) Type I

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 810 PF02190: LON_substr_bdg" amino acids 13 to 209 (197 residues), 189 bits, see alignment E=3e-59 TIGR00763: endopeptidase La" amino acids 15 to 776 (762 residues), 972.8 bits, see alignment E=8.1e-297 PF07728: AAA_5" amino acids 358 to 491 (134 residues), 38.6 bits, see alignment E=3.2e-13 PF00004: AAA" amino acids 358 to 496 (139 residues), 78.4 bits, see alignment E=2.1e-25 PF22667: Lon_lid" amino acids 518 to 569 (52 residues), 74.1 bits, see alignment 1.7e-24 PF05362: Lon_C" amino acids 576 to 778 (203 residues), 303 bits, see alignment E=2.9e-94 PF13541: ChlI" amino acids 619 to 746 (128 residues), 33.1 bits, see alignment E=1.4e-11

Best Hits

Swiss-Prot: 68% identical to LON_ECOLI: Lon protease (lon) from Escherichia coli (strain K12)

KEGG orthology group: K01338, ATP-dependent Lon protease [EC: 3.4.21.53] (inferred from 86% identity to aav:Aave_1457)

MetaCyc: 68% identical to Lon protease (Escherichia coli K-12 substr. MG1655)
Endopeptidase La. [EC: 3.4.21.53]

Predicted SEED Role

"ATP-dependent protease La (EC 3.4.21.53) Type I" in subsystem Proteasome bacterial or Proteolysis in bacteria, ATP-dependent (EC 3.4.21.53)

Isozymes

Compare fitness of predicted isozymes for: 3.4.21.53

Use Curated BLAST to search for 3.4.21.53

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (810 amino acids)

>GFF4044 ATP-dependent protease La (EC 3.4.21.53) Type I (Hydrogenophaga sp. GW460-11-11-14-LB1)
MSGQTPLPSTPVELPLLPLRDVVVFPHMVIPLFVGRPKSIKALESAMEAERRIMLVAQKA
AAKDEPSPDDMFEMGCVATILQMLKLPDGTVKVLVEGVQRAKVLTIRDGENHFIASVNPV
DPAVERADMQSNELEALRRAVMQQFDQYVKLNKKIPSEILTSISSIDDPGRLADTIAAHL
PLKLESKQVVLDLDPVNKRLENLFEQLEREVDILNVDKKIRGRVKRQMEKNQRDFYLNEQ
VKAIQKELGEGEEGADIEEIEKKIKAAKMPAEARKKADAEFKKLKLMSPMSAEATVVRNY
IDALVGLPWSKKTKIKHDLAHAEAVLNEDHYGLEKVKDRILEYLAVQQRVDKVKAPILCL
VGPPGVGKTSLGQSVAKATGRKYVRMALGGMRDEAEIRGHRRTYIGAMPGKVLQSLNKVG
TRNPLFLLDEIDKLGTDFRGDPSSALLEVLDPEQNHTFGDHYIEVDFDLSDVMFVATSNS
MNIPPALLDRMEVIRLSGYTEDEKTNIAIRYLLPKQIKNNGIREGELDVRDDAVRDIVRY
YTREAGVRSLERELSKICRKVVKGLLLKKYSPTVVVSADNLSEFLGVRKYSYGRAEAQNQ
VGQVVGLAWTEVGGDLLTIEVAIMPGKGVITRTGSLGDVMKESVEAARTVVRSRARMLGI
KDEQFDKRDIHIHVPDGATPKDGPSAGAAMTTALVSSLTGIPVRADVAMTGEITLRGEVT
AIGGLKEKLLAALRGGIKTVIIPEENVKDLAEIPDNVKQGLEIVPVKWIDKVLAIALERQ
PVPLTEEEVAAQIAAASVSKPAASPDAVKH