Protein Info for GFF4043 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: ATP-dependent Clp protease ATP-binding subunit ClpX

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 421 TIGR00382: ATP-dependent Clp protease, ATP-binding subunit ClpX" amino acids 9 to 412 (404 residues), 657.8 bits, see alignment E=3.4e-202 PF06689: zf-C4_ClpX" amino acids 14 to 49 (36 residues), 70.1 bits, see alignment 4.6e-23 PF00493: MCM" amino acids 70 to 196 (127 residues), 25.3 bits, see alignment E=3.1e-09 PF07724: AAA_2" amino acids 115 to 312 (198 residues), 119.7 bits, see alignment E=5.3e-38 PF07728: AAA_5" amino acids 117 to 194 (78 residues), 28.4 bits, see alignment E=6.2e-10 PF00004: AAA" amino acids 118 to 235 (118 residues), 58.5 bits, see alignment E=4.1e-19 PF10431: ClpB_D2-small" amino acids 318 to 390 (73 residues), 41.4 bits, see alignment E=4.7e-14

Best Hits

Swiss-Prot: 87% identical to CLPX_ACIAC: ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) from Acidovorax citrulli (strain AAC00-1)

KEGG orthology group: K03544, ATP-dependent Clp protease ATP-binding subunit ClpX (inferred from 87% identity to aaa:Acav_1497)

MetaCyc: 70% identical to ATP-dependent Clp protease ATP-binding subunit ClpX (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"ATP-dependent Clp protease ATP-binding subunit ClpX" in subsystem Proteasome bacterial or Proteolysis in bacteria, ATP-dependent

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (421 amino acids)

>GFF4043 ATP-dependent Clp protease ATP-binding subunit ClpX (Hydrogenophaga sp. GW460-11-11-14-LB1)
MADKKAASSEKALYCSFCGKSQHEVKKLIAGPSVFICDECIDLCNDIIRDELPGLESVKA
SGDDVPTPAQLKANLDNYVIGQQAAKRALAVAVYNHYKRLRHKVAARKDDVELAKSNILL
IGPTGSGKTLLAQTMARMLNVPFVMADATTLTEAGYVGEDVENIVAKLLQSCNYDVERAQ
QGIVYIDEIDKITRKSDNPSITRDVSGEGVQQALLKLVEGTMASVPPQGGRKHPNQDFLQ
VDTTNILFICGGAFAGLEKIIENRTEASGIGFGAVVKSKKQRSITEAFKEIEPEDLIKFG
LIPELVGRVPVIAALSELSEDALVEILTEPKNAVVKQFSRLLAMEGAELEVRPAALKAIA
RKALARKTGARGLRSILENALIDTMFDLPGMSNVEKVVVDESTIEESKPPLLVYREAAKQ
A