Protein Info for GFF4032 in Variovorax sp. SCN45

Annotation: Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 644 transmembrane" amino acids 15 to 34 (20 residues), see Phobius details amino acids 46 to 63 (18 residues), see Phobius details amino acids 69 to 86 (18 residues), see Phobius details amino acids 234 to 253 (20 residues), see Phobius details amino acids 265 to 287 (23 residues), see Phobius details amino acids 580 to 598 (19 residues), see Phobius details amino acids 604 to 623 (20 residues), see Phobius details TIGR01525: heavy metal translocating P-type ATPase" amino acids 48 to 621 (574 residues), 579.8 bits, see alignment E=1.1e-177 TIGR01494: HAD ATPase, P-type, family IC" amino acids 71 to 600 (530 residues), 259.6 bits, see alignment E=6.9e-81 PF00122: E1-E2_ATPase" amino acids 98 to 293 (196 residues), 179.4 bits, see alignment E=5.2e-57 PF00702: Hydrolase" amino acids 313 to 531 (219 residues), 128.7 bits, see alignment E=3.8e-41

Best Hits

KEGG orthology group: K01534, Cd2+/Zn2+-exporting ATPase [EC: 3.6.3.3 3.6.3.5] (inferred from 66% identity to ccr:CC_2726)

Predicted SEED Role

"Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4)" (EC 3.6.3.3, EC 3.6.3.4, EC 3.6.3.5)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.3, 3.6.3.4, 3.6.3.5

Use Curated BLAST to search for 3.6.3.3 or 3.6.3.4 or 3.6.3.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (644 amino acids)

>GFF4032 Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4) (Variovorax sp. SCN45)
LTAGWLIERSAAGPAWLPTATYIAAYFFGGFYTVKEAFENLKARRFEIDTLMLVAALGAA
ALGKWAEGALLLFLFSLGHSLEHYAMGRARKAIEALAKLAPETATVRRDSGTEEVAVEQL
KVGDVVVVRPNERLPADGVVVVGTSSVNQAPVTGESVPVDKRPVDDIKAALAAFDRVAPE
HRVFAGTINGSGAIEVTVARRAEQSTMARVVKMVTEAEAQRSPTQQFTERFERIFVPAVL
ALVVLLLFAGFVIDEPFSDTFYRAMAVLVAASPCALAISVPSAVLSGVARAGRGGVLVKG
GGPLENLGTLTSIAFDKTGTLTEGKPKLTDAVAMQGVTDDELLAVALAVEEHSDHPLATA
IVSGARERLGDHAKTVTASDVKSITGRGVRAQVDGELVYIGKPILFSELPGSSLPQDVKE
TNDKLVASGRTTMVVRKGERYLGVIAVMDTPRPVAAQVMAELRALGIERLIMISGDNQQV
ADAVAKSVGLTEARGDLMPEQKVDAIKALRKEHGKVAMVGDGVNDAPAMANSTVGIAMGA
AGSDVALETADVALMADDLTQLPFAVGLSRSTSRIIKQNLYVSLGVVAVLIPATIFGLNI
GTAVLFHEGSTLLVVVNALRLLAYKKGTFQAPQIATAVPAGATS