Protein Info for GFF4031 in Pseudomonas sp. DMC3

Annotation: Adaptive-response sensory-kinase SasA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 984 transmembrane" amino acids 6 to 27 (22 residues), see Phobius details amino acids 47 to 67 (21 residues), see Phobius details amino acids 73 to 93 (21 residues), see Phobius details amino acids 114 to 136 (23 residues), see Phobius details amino acids 155 to 173 (19 residues), see Phobius details amino acids 185 to 209 (25 residues), see Phobius details amino acids 241 to 260 (20 residues), see Phobius details amino acids 275 to 298 (24 residues), see Phobius details amino acids 325 to 354 (30 residues), see Phobius details amino acids 376 to 392 (17 residues), see Phobius details amino acids 398 to 422 (25 residues), see Phobius details amino acids 430 to 451 (22 residues), see Phobius details amino acids 471 to 493 (23 residues), see Phobius details TIGR00229: PAS domain S-box protein" amino acids 633 to 748 (116 residues), 38.5 bits, see alignment E=5.7e-14 PF00989: PAS" amino acids 635 to 678 (44 residues), 27.2 bits, see alignment (E = 6.6e-10) PF00512: HisKA" amino acids 758 to 819 (62 residues), 42.4 bits, see alignment 1.2e-14 PF02518: HATPase_c" amino acids 865 to 975 (111 residues), 86.8 bits, see alignment E=2.8e-28

Best Hits

KEGG orthology group: K00936, [EC: 2.7.3.-] (inferred from 98% identity to pfo:Pfl01_4807)

Predicted SEED Role

"Two-component sensor CbrA: intrcellular carbon:nitrogen balance"

Isozymes

Compare fitness of predicted isozymes for: 2.7.3.-

Use Curated BLAST to search for 2.7.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (984 amino acids)

>GFF4031 Adaptive-response sensory-kinase SasA (Pseudomonas sp. DMC3)
MPMSFSLTQMILISAAYLAALFGVAWVSERGMIPRAIIRHPLTYTLSLGVYASAWAFYGT
VGLAYQYGYGFLSSYLGVSGAFLLAPVLLYPILKITRTYQLSSLADLFAFRFRSTWAGAL
TTIFMLIGVLPLLALQIQAVADSIGILTGERVQSRVALAFCALIILFTIFFGSRHIATRE
KHEGLVFAIAFESIIKLIALGGVGLYALYGVFDGPQQLELWLLQNQTALAALHTPLQEGP
WRTLLLVFFASAIVMPHMYHMTFTENLNPRSLVSASWGLPLFLLLMSLAVPLILWAGLKL
GATTDPEYFTLGIGIAANSKPLALLAYVGGLSAASGLIIVTTLALSGMALNHLVLPLYQP
PAEGNIYRWLKWTRRALIVAIIMAGFGFYLMLGVEQDLANLGIVAFVATLQFLPGVLSVL
YWPTANRRGFIAGLLAGITVWVVTMLLPLVGNLQGFYIPLLNMIYVLDDTSWHMAAIASL
AANVLMFTLISLFTNASPEEASAAEACAVDNVRRPQRRELHAASPQEFATQLAKPLGAKA
AQKEVEQALRDLYLPFDERRPYALRRLRDRIEANLSGLMGPSVAQDMVETFLPYKAGGEN
YVTEDIHFIESRLEDYHSRLTGLAAELDALRRYHRQTLQELPMGVCSLAKDQEILMWNKA
MEELTGIAAQRVVGSRLSTIANPWKELLQGFINLPDEHLHKQHLALDGQTRWLNLHKAAI
DEPLAPGNSGLVLLVEDLTETQMLEDKLVHSERLASIGRLAAGVAHEIGNPITGIACLAQ
NLREEREDDGELTEISGQILEQTKRVSRIVQSLMSFAHAGSHQHNDEPVCLAEVAQDAIG
LLALNRRNFEVQFYNLCDPDHWVEGDPQRLAQVLINLLSNARDASPPHSAVRVKSEAGEH
TVDLIVEDEGSGIPSSIMDRLFEPFFTTKDPGEGTGLGLALVYSIVEEHYGQITIDSPAD
VQSQRGTRIRVTLPRHVEATSAVN