Protein Info for GFF4030 in Xanthobacter sp. DMC5

Annotation: Cation/acetate symporter ActP

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 554 signal peptide" amino acids 1 to 28 (28 residues), see Phobius details transmembrane" amino acids 43 to 62 (20 residues), see Phobius details amino acids 85 to 106 (22 residues), see Phobius details amino acids 112 to 132 (21 residues), see Phobius details amino acids 155 to 176 (22 residues), see Phobius details amino acids 188 to 211 (24 residues), see Phobius details amino acids 221 to 241 (21 residues), see Phobius details amino acids 271 to 295 (25 residues), see Phobius details amino acids 307 to 332 (26 residues), see Phobius details amino acids 364 to 392 (29 residues), see Phobius details amino acids 413 to 432 (20 residues), see Phobius details amino acids 438 to 460 (23 residues), see Phobius details amino acids 471 to 490 (20 residues), see Phobius details amino acids 509 to 527 (19 residues), see Phobius details TIGR00813: transporter, solute:sodium symporter (SSS) family" amino acids 72 to 478 (407 residues), 195.2 bits, see alignment E=1e-61 PF00474: SSF" amino acids 72 to 478 (407 residues), 375.2 bits, see alignment E=2e-116

Best Hits

Swiss-Prot: 63% identical to ACTP_PECCP: Cation/acetate symporter ActP (actP) from Pectobacterium carotovorum subsp. carotovorum (strain PC1)

KEGG orthology group: None (inferred from 80% identity to mno:Mnod_3160)

MetaCyc: 63% identical to acetate/glycolate:cation symporter (Escherichia coli K-12 substr. MG1655)
RXN0-1981; RXN0-5111; TRANS-RXN0-576

Predicted SEED Role

"Acetate permease ActP (cation/acetate symporter)" in subsystem Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (554 amino acids)

>GFF4030 Cation/acetate symporter ActP (Xanthobacter sp. DMC5)
MRWTPLVRAASAALTFALACAASPAWAAGAIEGGARQPINPVAISLFLLFVAVSLGVTWW
AARKGTGGAQDYYAAGGGLSGFKNGLAIAGDYTSAATFLGVTALVYTSGYDGMIYAIGFL
VGFPMILFLIAEPLRNLGRFTFADVAAYRLSEVPVRVVAATNTLVIVLLYLIAQMVGAGK
LIELLFGLPYWVAVGLVGLLMMAYVTFGGMLATTWVQIIKAVLLLTGSVVMSALILGHFG
FSLEALFAHAAALHPKGAAIMAPGGLVKDPISAISLGVALVFGTAGLPHILMRFFTVADA
RQARTSVLVATGFISVFYTLLFVLGFGAIAFVMSDPSFKDATGAVVGGTNMVALHLANAL
GGSVLLGFISAVAFATILAVVSGLTIAGASAASHDLYARVIRRGKASEQEEVRVSKAAAV
VISLLAMGLGLLFENQNIAFMVGLVFAIAASANFPVIVLSVSWPGLTTRGAVTGSVTGLV
VALVLVALSPSVWTTTLKLGPAPFPYDNPALFSVPLAFLVSIVVSLADRSTAAGRVRAAF
AAQHVRAQTGFAVD