Protein Info for GFF403 in Variovorax sp. SCN45

Annotation: Inactive (p)ppGpp 3'-pyrophosphohydrolase domain / GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase I

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 746 PF13328: HD_4" amino acids 41 to 201 (161 residues), 152.1 bits, see alignment E=2.7e-48 TIGR00691: RelA/SpoT family protein" amino acids 52 to 740 (689 residues), 542.4 bits, see alignment E=7.8e-167 PF04607: RelA_SpoT" amino acids 260 to 374 (115 residues), 137.1 bits, see alignment E=7.8e-44 PF02824: TGS" amino acids 418 to 476 (59 residues), 75.9 bits, see alignment 5.1e-25 PF13291: ACT_4" amino acids 672 to 745 (74 residues), 52.7 bits, see alignment E=1.4e-17

Best Hits

KEGG orthology group: K00951, GTP pyrophosphokinase [EC: 2.7.6.5] (inferred from 95% identity to vpe:Varpa_3336)

Predicted SEED Role

"GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase I" in subsystem Stringent Response, (p)ppGpp metabolism (EC 2.7.6.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.6.5

Use Curated BLAST to search for 2.7.6.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (746 amino acids)

>GFF403 Inactive (p)ppGpp 3'-pyrophosphohydrolase domain / GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase I (Variovorax sp. SCN45)
VKRDSSSLQTSPVSDMAMVDYPLSAATAERSPVMENMLARARAFAEPLIADETLDTGENT
LAHADAVAAIVAKMGGSEAMQAASYLVYACQHLNRPQEVIAKVFGDNFAALAVETTKLVR
VQEQARSAAQGHHIEGAGAQTENVRKMLLAFSRDLRVVMLRLASRLQTLRHAAASKQPAP
EGVARESLQVFAPLANRLGIWQVKWEIEDLSFRFLEPETYKLIARLLDEKRIEREGHVEQ
LRSQLERELQNEGVKATVQGRPKNIYSIVKKMRGKSLDFAQVFDILALRVVVSDVKDCYA
ALAWVHSHFQPIDEEFDDYIARPKPNGYQSLHTVVRELVDGKPGKPIEIQIRTEEMHDHA
EHGVAAHWAYKEAGHKGYAGVWASGEYDAKIAVLRQLLAWERDLSGGLQGQGLFDDRIYV
LTPDAAIVELPQGATPVDFAYTVHTTLGHRCRGARVDGAMVPLNTPLSNGQTVEIIAAKE
GGPSRDWLNAELGYLASHRARAKVRAWFNAQITHETVARGREAVEKLLQREGKTAVRLED
LASQLGFKSADHLFEVVGKDEFSLRNIETLLRPPEPAPGPDDGVLIKKARGSEKSGKGGV
LVVGVSSLMTQLAKCCKPAPPDSIRGFVTRGHGVSVHRSDCSNFRTMASKDNERVIDVEW
GAPKKGAEAPVYAVDVAVEAADRQGLLRDISDVFAREKMNVIGVQTQSIKGTAWMTFTVE
IADAARLTQVLGVVTAVIGVRSARRR