Protein Info for GFF4009 in Variovorax sp. SCN45

Annotation: D-amino acid dehydrogenase (EC 1.4.99.6)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01266: DAO
  • amino acids 2 to 399 (398 residues), 239.9 bits, E=2.2e-74
• PF13450: NAD_binding_8
  • amino acids 5 to 32 (28 residues), 29.5 bits, see alignment (E = 2.6e-10)

Best Hits

Swiss-Prot: 36% identical to DADA_BRADU: D-amino acid dehydrogenase (dadA) from Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)

KEGG orthology group: K00285, D-amino-acid dehydrogenase [EC: 1.4.99.1] (inferred from 89% identity to vap:Vapar_1545)

Predicted SEED Role

"D-amino acid dehydrogenase small subunit (EC 1.4.99.1)" in subsystem Pyruvate Alanine Serine Interconversions or Respiratory dehydrogenases 1 (EC 1.4.99.1)

KEGG Metabolic Maps

• Nitrogen metabolism
• Phenylalanine metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.4.99.1

Use Curated BLAST to search for 1.4.99.1

Protein Sequence (403 amino acids)

>GFF4009 D-amino acid dehydrogenase (EC 1.4.99.6) (Variovorax sp. SCN45)
MHVCVLGAGIVGLATAWQLERQGHQVTVIDRATPGAGASGGNGAQLSYSYVQPLADPSIW
KQLPKLLLSPTSPLKLRPQLDPLQWRWGMAFLAACNARTSRDTTARLLALAAESRAGFES
MQADISPDCDFSATGKLVLYASAASLEGARAQVELQRTMGSEQRLVTPDECIAIEPALAG
YRGQMAGAVYTPSECAADCLKVCAELMRALGARGVRFMLGTDVHGFARDDKRIAAVRTSG
GDVEAEAFVMALGTASHKLGRALGAYLPVYPLKGYSITVDVDPAPGAAPHVNVTDSARKV
VFARIGSRLRVAGMAELVGHDASIPAARIETLEAATRALFPHASRFEELHLWTGMRPATP
KGLPIIGQLESAPPNMLFNTGHGALGFTLAFGSAQRIARALPS