Protein Info for GFF3983 in Pseudomonas sp. DMC3

Annotation: Glutamate 5-kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00696: AA_kinase
  • amino acids 11 to 239 (229 residues), 164.7 bits, E=3.1e-52
• TIGR01027: glutamate 5-kinase
  • amino acids 11 to 371 (361 residues), 433.8 bits, E=2.8e-134
• PF01472: PUA
  • amino acids 282 to 352 (71 residues), 75.9 bits, E=2e-25

Best Hits

Swiss-Prot: 99% identical to PROB_PSEPF: Glutamate 5-kinase (proB) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K00931, glutamate 5-kinase [EC: 2.7.2.11] (inferred from 99% identity to pfs:PFLU5168)

MetaCyc: 44% identical to glutamate 5-kinase (Escherichia coli K-12 substr. MG1655)
Glutamate 5-kinase. [EC: 2.7.2.11]

Predicted SEED Role

"Glutamate 5-kinase (EC 2.7.2.11) / RNA-binding C-terminal domain PUA" in subsystem Proline Synthesis (EC 2.7.2.11)

MetaCyc Pathways

• L-citrulline biosynthesis (7/8 steps found)
• L-proline biosynthesis I (from L-glutamate) (4/4 steps found)
• L-ornithine biosynthesis II (3/3 steps found)
• superpathway of L-citrulline metabolism (9/12 steps found)
• L-N^δ-acetylornithine biosynthesis (5/7 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Urea cycle and metabolism of amino groups

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.2.11

Protein Sequence (373 amino acids)

>GFF3983 Glutamate 5-kinase (Pseudomonas sp. DMC3)
MMRSKVTGAQRWVVKIGSALLTADGKGLDRAAMSVWVEQMVALHEAGVELVLVSSGAVAA
GMSRLGWTARPSAMHELQAAAAIGQMGLVQAWESSFAEHGRHTAQILLTHDDLSDRKRYL
NARSTLRALVELKVIPVINENDTVVTDEIRFGDNDTLAALVANLVEADLLVILTDRDGMF
DADPRNNPDAQLIYEARADDPTLDAVAGGTGGALGRGGMQTKLRAARLAARSGAHTIIVG
GRLERVLDRLKAGERIGTLLSPERGMLAARKQWLAGHLQTRGTLVLDEGAVSALSKGNKS
LLPVGVKLVQGSFRRGEMVVCVAPDGREIARGLANYSALEAQKIIGQSSDAIVGLLGYMA
EPELVHRDNLILV