Protein Info for GFF3980 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Vitamin B12 ABC transporter, B12-binding component BtuF

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 289 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details PF01497: Peripla_BP_2" amino acids 43 to 255 (213 residues), 103.4 bits, see alignment E=6.5e-34

Best Hits

KEGG orthology group: K02016, iron complex transport system substrate-binding protein (inferred from 68% identity to pna:Pnap_2364)

Predicted SEED Role

"Vitamin B12 ABC transporter, B12-binding component BtuF" in subsystem Coenzyme B12 biosynthesis

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (289 amino acids)

>GFF3980 Vitamin B12 ABC transporter, B12-binding component BtuF (Hydrogenophaga sp. GW460-11-11-14-LB1)
MPLRFWRLLALGCLIGGAAHAADVTLTDDRGVTVTLAQSPQRIVSVLPSLTETVCELGQC
HRLVGVDRYSNYPASVRGLPQVGGGIDPNIEAIVALKPDVVLMATSSRGVQRLESLGVKV
LAMEPRSSADAQRVMGKLGQLLEVPDAQRIWRAIDAGVSAVAQSLPPGARKLRVYYEVSA
GGYAAGTGSFIGEMMARLGVQNIVAPALGPFPRVNPEYVVRANPDLIMIGARNVQGLETR
PGWRGIRALRERRLCVFTAEESDVLVRPGPRMAEAARLMARCITEKGLR