Protein Info for GFF3968 in Variovorax sp. SCN45

Annotation: Indolepyruvate oxidoreductase subunit IorB II (EC 1.2.7.8)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 501 transmembrane" amino acids 159 to 175 (17 residues), see Phobius details PF01558: POR" amino acids 16 to 206 (191 residues), 30.1 bits, see alignment E=5.2e-11 PF20169: DUF6537" amino acids 251 to 461 (211 residues), 158.8 bits, see alignment E=1.7e-50

Best Hits

KEGG orthology group: K00180, indolepyruvate ferredoxin oxidoreductase, beta subunit [EC: 1.2.7.8] (inferred from 93% identity to vpe:Varpa_1647)

Predicted SEED Role

"Indolepyruvate oxidoreductase subunit IorB II (EC 1.2.7.8)" in subsystem Aromatic amino acid interconversions with aryl acids (EC 1.2.7.8)

Isozymes

Compare fitness of predicted isozymes for: 1.2.7.8

Use Curated BLAST to search for 1.2.7.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (501 amino acids)

>GFF3968 Indolepyruvate oxidoreductase subunit IorB II (EC 1.2.7.8) (Variovorax sp. SCN45)
MTSTAQPIKIAILAMGGEGGGVLADWIVDMGEANGYVAQTTSVPGVAQRTGATIYYVELY
PTAQAEADGGLPILALMPLPGDVDVVLASELMEAGRAVQRGLVTRDRTTLIASTHRVFSI
AEKSAMGDGRVDSDQLLAHTAKAARRFIRFDMAQAAEASGSVISAVLFGALAGSGVLPFS
RAQFEATIERGGVGVKPSLKAFGAAFARAQGGDDGEPTSEAASAAPAPQPRHPAVRALVE
RVQRDFPVAAQDLLLEGVRRLIDYQDPAYAGLYLDRMAAVAALPASDHRLLRETARHLAL
WMSYEDTARVAALKTRATRFERVRGEARVQPGQVLAINEYMHPRLQEICETLPGGVGRWL
MNSTLPKKLVERFTQHGRVIQTSSLRGYLMLRMVAACKRWRLSTMRYAEENRRIEEWLQR
IAATAQRNPELAVELAQCQRLVKGYSDTHERGIRNYDTVMRAFERAGAALAPATLRELRD
AALADEHGHKLQAALAQHALV