Protein Info for GFF3946 in Xanthobacter sp. DMC5

Annotation: Gamma-glutamyl phosphate reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00171: Aldedh
  • amino acids 31 to 303 (273 residues), 52.2 bits, E=2e-18
• TIGR00407: glutamate-5-semialdehyde dehydrogenase
  • amino acids 35 to 429 (395 residues), 442.5 bits, E=6.6e-137

Best Hits

Swiss-Prot: 74% identical to PROA_RHOP2: Gamma-glutamyl phosphate reductase (proA) from Rhodopseudomonas palustris (strain HaA2)

KEGG orthology group: K00147, glutamate-5-semialdehyde dehydrogenase [EC: 1.2.1.41] (inferred from 86% identity to xau:Xaut_2058)

MetaCyc: 44% identical to glutamate-5-semialdehyde dehydrogenase (Escherichia coli K-12 substr. MG1655)
Glutamate-5-semialdehyde dehydrogenase. [EC: 1.2.1.41]

Predicted SEED Role

"Gamma-glutamyl phosphate reductase (EC 1.2.1.41)" in subsystem Proline Synthesis (EC 1.2.1.41)

MetaCyc Pathways

• L-proline biosynthesis I (from L-glutamate) (4/4 steps found)
• L-ornithine biosynthesis II (3/3 steps found)
• L-citrulline biosynthesis (6/8 steps found)
• superpathway of L-citrulline metabolism (8/12 steps found)
• L-N^δ-acetylornithine biosynthesis (4/7 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Urea cycle and metabolism of amino groups

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.2.1.41

Protein Sequence (440 amino acids)

>GFF3946 Gamma-glutamyl phosphate reductase (Xanthobacter sp. DMC5)
MSTASALRTDAAATAGRLVPPAELEAVMLAMGKRARAASRSLALAAPEAKTQALKAAAAA
IRAASGEILAANAIDVAKAKAEGMTGSFLDRLTLTPARVEAMAEGVEQVAELADPIGLVT
ERWTRPNGLIIERVRTPLGVIGVIYESRPNVTADAAALCLRAGNAVILRGGSDSLNSSGA
IHRAFVAGLTAAGLPEDAVQFVNVPDRAAVGHMLAGLGNTVDVIVPRGGKSLVARVQSDA
RVPVFAHLDGINHVYVDKAANLDMAKTVVLNAKMRRTGVCGAAETLLIDRAVAGTHLKPL
VEMLIDAGCEIRGDAEVQAVDARVKPALPEDWDTEYLDAIIAAKVVDGVGDAIAHIEAHG
SHHTDSIVTEDAAAADRFLAEVDSAIVLHNASTQFADGGEFGFGAEIGIATGRMHARGPV
GAEQLTSFKYRIHGSGQTRP