Protein Info for PS417_20190 in Pseudomonas simiae WCS417

Annotation: nitrate reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 503 transmembrane" amino acids 34 to 56 (23 residues), see Phobius details amino acids 62 to 85 (24 residues), see Phobius details amino acids 106 to 126 (21 residues), see Phobius details amino acids 146 to 167 (22 residues), see Phobius details amino acids 176 to 193 (18 residues), see Phobius details amino acids 211 to 233 (23 residues), see Phobius details amino acids 249 to 276 (28 residues), see Phobius details amino acids 296 to 319 (24 residues), see Phobius details amino acids 339 to 358 (20 residues), see Phobius details amino acids 364 to 388 (25 residues), see Phobius details amino acids 421 to 442 (22 residues), see Phobius details amino acids 454 to 474 (21 residues), see Phobius details PF02133: Transp_cyt_pur" amino acids 20 to 455 (436 residues), 165.7 bits, see alignment E=8.6e-53

Best Hits

KEGG orthology group: None (inferred from 98% identity to pfs:PFLU4512)

Predicted SEED Role

"Cytosine/purine/uracil/thiamine/allantoin permease family protein" in subsystem Purine Utilization

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7U4K9 at UniProt or InterPro

Protein Sequence (503 amino acids)

>PS417_20190 nitrate reductase (Pseudomonas simiae WCS417)
MNNKNNHKSIRKIETNGVEQIPDHERTAGPKDLFRLIFGGANTFATAVLGSFPVLFGLSF
QAGVWAIVLGVLVGALILAPMGLFGPINGTNNAVSSGAHFGVHGRIVGSFLSLLTAIAFF
SLSVWSSGDALVGGAKRLIGLPETDLTLGLAYGVFAILVLTVCIYGFRFMLWVNRIAVWA
ASLLFLLGIFAFAPEFDSHFAGTVALGQTGFWAAFIGAALVAMSNPISFGAFLGDWSRYI
PRETPKIRIMLAVVLAQIATLIPFLFGLATATIVAIKAPDYIAANNYVGGLLAVAPSWFF
LPVCLIAVIGGMSTGTTSLYGTGLDMSSVFPRLLSRVKATLLIGVMSIAFIFIGRFAANL
VQSVSTFAVLIITCTTPWMVMMIIGLIVRRGFYCPDDLQVFTRGETGGRYWFSHGWNWRG
LGAWIPSALMGLCFVNLPGQFVGPLGNLAEGIDISLPVTLGLASLMYLTLLRVFPEPAAV
YGPTDSRSKRTDARVKPALQQAA