Protein Info for GFF3930 in Variovorax sp. SCN45

Annotation: Peptidoglycan lipid II flippase MurJ

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 555 transmembrane" amino acids 39 to 58 (20 residues), see Phobius details amino acids 64 to 83 (20 residues), see Phobius details amino acids 128 to 151 (24 residues), see Phobius details amino acids 168 to 187 (20 residues), see Phobius details amino acids 197 to 217 (21 residues), see Phobius details amino acids 224 to 244 (21 residues), see Phobius details amino acids 279 to 299 (21 residues), see Phobius details amino acids 319 to 339 (21 residues), see Phobius details amino acids 356 to 381 (26 residues), see Phobius details amino acids 396 to 416 (21 residues), see Phobius details amino acids 428 to 447 (20 residues), see Phobius details amino acids 453 to 475 (23 residues), see Phobius details amino acids 487 to 509 (23 residues), see Phobius details amino acids 523 to 547 (25 residues), see Phobius details TIGR01695: murein biosynthesis integral membrane protein MurJ" amino acids 40 to 548 (509 residues), 412.7 bits, see alignment E=1.1e-127 PF03023: MurJ" amino acids 66 to 510 (445 residues), 394.9 bits, see alignment E=4.3e-122 PF13440: Polysacc_synt_3" amino acids 275 to 382 (108 residues), 27.9 bits, see alignment E=1.4e-10

Best Hits

KEGG orthology group: K03980, virulence factor (inferred from 90% identity to vpe:Varpa_1610)

Predicted SEED Role

"Proposed peptidoglycan lipid II flippase MurJ" in subsystem ZZ gjo need homes

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (555 amino acids)

>GFF3930 Peptidoglycan lipid II flippase MurJ (Variovorax sp. SCN45)
MSEDDAAKPSIIGRVGPFAEFAPESARSGARRGLHSAPVSLLKSASTVSLLTLASRITGL
VRDVLFASVFGVSALTDAFNVAFRIPNLFRRVFGEGAFSQAFVPVLAGRKTEAGEEGARQ
LIDHVATLLTWTLVVVCVIGVVAAPLMVWAMASGLAGFDSAVVMTRWMFPYIGFMSLVAL
AGGILNTWRKFAVPAASPVLLNIALILSISVGAPLFRRYGIEPIYAQCVGVLVGGMLQLA
LQIPALRSLGLMPRVGASFRALRAAWTDPTTRKVMKLMLPALLGVSVAQISLLINTQIAS
HLAAGSVTWVVNADRLMEFPTAMLGVALGVVLMPQLAGARAAKDDARYSSLLDWGLRLVV
LLSAPCAVALLLFAQPLVAVLFHNGAYAGDDVRRTALALTGYGVGLIGIVAVKVLAPGYY
AKQDTRTPMLIAVGVLVFTQVLNFFLVPVLQHAALTLTIAIGALVNATWLLVGLIRRGSY
KPEPGWGKFALQVLAGTLVLAALLVWGSAHFDWIGLREQRLLRIGLLAALIAASVVLYFG
VLAAVGVRLRSFLRR