Protein Info for GFF3921 in Variovorax sp. SCN45
Annotation: Tryptophan 2,3-dioxygenase (EC 1.13.11.11)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 82% identical to T23O_POLSJ: Tryptophan 2,3-dioxygenase (kynA) from Polaromonas sp. (strain JS666 / ATCC BAA-500)
KEGG orthology group: K00453, tryptophan 2,3-dioxygenase [EC: 1.13.11.11] (inferred from 95% identity to vpe:Varpa_1601)Predicted SEED Role
"Tryptophan 2,3-dioxygenase (EC 1.13.11.11)" in subsystem Aromatic amino acid degradation or NAD and NADP cofactor biosynthesis global (EC 1.13.11.11)
MetaCyc Pathways
- NAD de novo biosynthesis II (from tryptophan) (8/9 steps found)
- superpathway of NAD biosynthesis in eukaryotes (11/14 steps found)
- L-tryptophan degradation I (via anthranilate) (3/3 steps found)
- 3-hydroxy-4-methyl-anthranilate biosynthesis II (4/5 steps found)
- L-tryptophan degradation to 2-amino-3-carboxymuconate semialdehyde (4/5 steps found)
- 3-hydroxy-4-methyl-anthranilate biosynthesis I (3/6 steps found)
- L-tryptophan degradation III (eukaryotic) (8/15 steps found)
- L-tryptophan degradation IX (5/12 steps found)
- superpathway of aromatic compound degradation via 3-oxoadipate (21/35 steps found)
- L-tryptophan degradation XII (Geobacillus) (4/12 steps found)
- L-tryptophan degradation XI (mammalian, via kynurenine) (9/23 steps found)
- superpathway of aromatic compound degradation via 2-hydroxypentadienoate (17/42 steps found)
KEGG Metabolic Maps
Isozymes
No predicted isozymesUse Curated BLAST to search for 1.13.11.11
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (286 amino acids)
>GFF3921 Tryptophan 2,3-dioxygenase (EC 1.13.11.11) (Variovorax sp. SCN45) MSPDKSPATSPENIVHEEKAQLDFSVSMSYGDYLHLDEILNAQHPLSPAHDEMLFIVQHQ TSELWMKLMLHELRAATACIAEEKLADAFKMLARVSRIMEQLVSAWTVLSTMTPPEYTAM RPYLANSSGFQSAQYRCIEFALGNKNAAMLKPHAHRADLLAQVEAAYRAPSLYDESLRLL ARHGLPVPADRLERDWTQPYEPSDGVEAAWLAVYRDPHAHWDLYQLGEKLTDLEDAFRLW RFRHVTTVERVIGFKRGTGGTGGVSYLRKMLEVVLFPEIWKLRTDL