Protein Info for GFF3920 in Hydrogenophaga sp. GW460-11-11-14-LB1
Annotation: Phosphoenolpyruvate synthase (EC 2.7.9.2)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 68% identical to PPSA_PSEAB: Phosphoenolpyruvate synthase (ppsA) from Pseudomonas aeruginosa (strain UCBPP-PA14)
KEGG orthology group: K01007, pyruvate, water dikinase [EC: 2.7.9.2] (inferred from 87% identity to adk:Alide2_1662)MetaCyc: 65% identical to phosphoenolpyruvate synthetase (Escherichia coli K-12 substr. MG1655)
Pyruvate, water dikinase. [EC: 2.7.9.2]
Predicted SEED Role
"Phosphoenolpyruvate synthase (EC 2.7.9.2)" in subsystem Glycolysis and Gluconeogenesis or Glycolysis and Gluconeogenesis, including Archaeal enzymes or Pyruvate metabolism I: anaplerotic reactions, PEP (EC 2.7.9.2)
MetaCyc Pathways
- superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (22/26 steps found)
- superpathway of glycolysis and the Entner-Doudoroff pathway (15/17 steps found)
- glycolysis II (from fructose 6-phosphate) (10/11 steps found)
- gluconeogenesis I (11/13 steps found)
- glycolysis I (from glucose 6-phosphate) (11/13 steps found)
- superpathway of anaerobic sucrose degradation (14/19 steps found)
- glycolysis V (Pyrococcus) (7/10 steps found)
- hexitol fermentation to lactate, formate, ethanol and acetate (13/19 steps found)
- superpathway of hexitol degradation (bacteria) (11/18 steps found)
- superpathway of N-acetylneuraminate degradation (13/22 steps found)
- gluconeogenesis II (Methanobacterium thermoautotrophicum) (9/18 steps found)
- Methanobacterium thermoautotrophicum biosynthetic metabolism (21/56 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 2.7.9.2
Use Curated BLAST to search for 2.7.9.2
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (797 amino acids)
>GFF3920 Phosphoenolpyruvate synthase (EC 2.7.9.2) (Hydrogenophaga sp. GW460-11-11-14-LB1) MSQLFGATDLVVPFEKLRMSDVESVGGKNASLGEMISQLPTGVKVPTGFATTAHAFREFL KHDGLTERINARLDQLDTEDVRALAQAGAEIRAMVENQPFPADLEKSIRDAFVTLSAGNA KASFAVRSSATAEDLPDASFAGQQETFLNVVGIEEVLHKMKEVFASLYNDRAISYRVHKG FAHADVALSAGVQRMVRSDTGAAGVMFTIDTESGFEEVVFITSSYGLGETVVQGAVNPDE FYVHKPMLKAGKRALIRRNLGSKLIQMVFSTPEEKAASGKLVKTVDVPVEQRNRYSLSDA DVEQLAKYALVIEQHYGRPMDIEWGKDGTDGQIYILQARPETVKSQSAGKTEVRYKLKGK GAVLASGRAIGQKVGTGPVRLVHNISEMDKVQPGDVLVTDMTDPNWEPVMKRASAIVTNR GGRTCHAAIIARELGIPAVVGCGDATDLLKDGTLVTVSCAEGDTGNIYDGLLETEITEVQ RGEMPALDVKIMMNVGNPQLAFDFAQIPNGGVGLARLEFIINNNIGVHPKAILDYPNVDA DLKKAVESVARGHASPRAFYVDKVAEGVATIAAAFWPKPVIVRLSDFKSNEYRKLVGGSR YEPEEENPMLGFRGAARYVSEEFREAFAMECEALKRVREDMGLTNVQVMVPFVRTLAQAE RVTGLLADKGLKRGVNDLKVIMMCEIPSNAVLAKQFLQFFDGFSIGSNDLTQLTLGLDRD SGLELLARDFDERDPAVKALLTMAIGACKAEGKYVGICGQGPSDHPDFAEWLRDEGISSI SLNPDSVIDTWKQLAKE