Protein Info for PS417_20040 in Pseudomonas simiae WCS417

Annotation: cobyric acid synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 483 PF13500: AAA_26" amino acids 3 to 230 (228 residues), 62.3 bits, see alignment E=8.7e-21 PF01656: CbiA" amino acids 4 to 230 (227 residues), 78.4 bits, see alignment E=7.2e-26 TIGR00313: cobyric acid synthase CobQ" amino acids 4 to 475 (472 residues), 484.1 bits, see alignment E=2.4e-149 PF07685: GATase_3" amino acids 250 to 433 (184 residues), 190.4 bits, see alignment E=4.1e-60

Best Hits

Swiss-Prot: 96% identical to COBQ_PSEFS: Cobyric acid synthase (cobQ) from Pseudomonas fluorescens (strain SBW25)

KEGG orthology group: K02232, adenosylcobyric acid synthase [EC: 6.3.5.10] (inferred from 96% identity to pfs:PFLU4486)

MetaCyc: 49% identical to CobQ (Pseudomonas denitrificans (nom. rej.))
Adenosylcobyric acid synthase (glutamine-hydrolyzing). [EC: 6.3.5.10]

Predicted SEED Role

"Cobyric acid synthase (EC 6.3.5.10)" (EC 6.3.5.10)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.5.10

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7UIT4 at UniProt or InterPro

Protein Sequence (483 amino acids)

>PS417_20040 cobyric acid synthase (Pseudomonas simiae WCS417)
MSTLMVQGTTSDAGKSTLVTALCRWLVRQGVAVVPFKPQNMALNSAVTAEGGEIGRAQAV
QAQAANLAPHTDMNPVLLKPNSDTGSQVIIHGRAVTTMNAVAYHDYKSIAMQAVLASHAR
LSQAYPVVMVEGAGSPAEINLRANDIANMGFAEAVDCPVLLIADINRGGVFAHLVGTLEL
LSPTEQARVKGFIINRFRGDIALLQPGLDWLEARTGKPVVGVLPYVMDLHLEAEDGIDRR
QTDKVAQVLKVVVPVLPRISNHTDFDPLRLHPQVDLQFIGPGQPIPAADLIILPGSKSVR
SDLSYLRTNGWDTAVARHLRYGGKVLGICGGLQMLGEQVHDPLGLEGPAGSSDGLGLLAF
STTLEEEKQLRNVRGRLLLEDAEVCGYEIHAGVTSGDALSNAAVLLDDGRTDGAQSADGQ
ILGTYLHGLFESPAACSALLRWAGLQDVQAVDYHALRERDIERLADLVENHLDTDLLRKL
CGG