Protein Info for GFF3885 in Variovorax sp. SCN45

Annotation: tRNA pseudouridine(38-40) synthase (EC 5.4.99.12)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00071: tRNA pseudouridine(38-40) synthase
  • amino acids 3 to 241 (239 residues), 204.9 bits, E=7.1e-65
• PF01416: PseudoU_synth_1
  • amino acids 8 to 101 (94 residues), 44.7 bits, E=8.3e-16
  • amino acids 142 to 248 (107 residues), 94.9 bits, E=2.1e-31

Best Hits

Swiss-Prot: 95% identical to TRUA_VARPS: tRNA pseudouridine synthase A (truA) from Variovorax paradoxus (strain S110)

KEGG orthology group: K06173, tRNA pseudouridine synthase A [EC: 5.4.99.12] (inferred from 95% identity to vap:Vapar_1417)

Predicted SEED Role

"tRNA pseudouridine synthase A (EC 4.2.1.70)" in subsystem tRNA processing (EC 4.2.1.70)

MetaCyc Pathways

• pseudouridine degradation (1/2 steps found)

KEGG Metabolic Maps

• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.70, 5.4.99.12

Use Curated BLAST to search for 4.2.1.70 or 5.4.99.12

Protein Sequence (270 amino acids)

>GFF3885 tRNA pseudouridine(38-40) synthase (EC 5.4.99.12) (Variovorax sp. SCN45)
VRLALGIRYNGQAYEGWQSQRSGRTVQDKLEAALSKFAAQPIGTLCAGRTDAGVHALMQV
VHFDTTVEREPFSWMRGPNRFLPDDIAVQWAQPVPDEFHCRASALARRYLYVLSQSPVRP
SLDTGRVGWSMHALDGDAMRAAAALLVGRHDFSSFRASACQARSPVKDLRRIEITRVGEG
ERCRWHFEFEADAFLHHMIRNLMGCLVRIGRGDEPVEWISQVLEARSRKVAAPTFSADGL
YFLGPLYDAKWGLPADATLQAGGAPYDGPP