Protein Info for PS417_19870 in Pseudomonas simiae WCS417

Annotation: flagellar rod assembly protein FlgJ

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 423 PF10135: Rod-binding" amino acids 57 to 108 (52 residues), 61.4 bits, see alignment 9.7e-21 TIGR02541: flagellar rod assembly protein/muramidase FlgJ" amino acids 207 to 408 (202 residues), 205.7 bits, see alignment E=7.1e-65 PF01832: Glucosaminidase" amino acids 271 to 409 (139 residues), 122.4 bits, see alignment E=1.9e-39

Best Hits

KEGG orthology group: K02395, flagellar protein FlgJ (inferred from 98% identity to pfs:PFLU4452)

Predicted SEED Role

"Flagellar protein FlgJ [peptidoglycan hydrolase] (EC 3.2.1.-)" in subsystem Flagellum (EC 3.2.1.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.-

Use Curated BLAST to search for 3.2.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7U1Z1 at UniProt or InterPro

Protein Sequence (423 amino acids)

>PS417_19870 flagellar rod assembly protein FlgJ (Pseudomonas simiae WCS417)
MAMDMRKSGISSTADSGSYSDLNRLNQLKVGDDKNSEGNMRKVAQEFESLFLSEMLKSMR
SATEALGKDNPMNTPAAKQYQEMYDQQLAVSMSREGGGIGLADVLMRQMQKNKPVEAQAA
TLQGPAAAEAVKKVDVPTEIAAGTQAVGPLGRSNGQRPLWAYRVAEPQAGAVASHSNDME
LMNQRRIALPSKLTDRLLAGIVPNAPVAIDAKSAPLRNSAADDNVVNSTARTFAVPSGRM
QVYGRAVAQPPLAPAKKAFSSQDEFVATMLPMAKAAAARIGVDPKYLVAQAALETGWGKS
VMRAEDGSSSHNLFGIKAGQSWQGGQARAITSEFRDGAMVKETAQFRSYSSYQDSFHDLV
TLLQSNDRYKEVVKSADNPEQFVRELQKAGYATDPAYASKISQIAKTMDSYQNYAAAGAT
THL