Protein Info for GFF3875 in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868

Annotation: GDP-mannose 4,6-dehydratase (EC 4.2.1.47)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01472: GDP-mannose 4,6-dehydratase
  • amino acids 3 to 353 (351 residues), 588.7 bits, E=1.8e-181
• PF01370: Epimerase
  • amino acids 5 to 252 (248 residues), 269.7 bits, E=4.2e-84
• PF16363: GDP_Man_Dehyd
  • amino acids 6 to 346 (341 residues), 521.2 bits, E=2.8e-160
• PF04321: RmlD_sub_bind
  • amino acids 6 to 162 (157 residues), 30.1 bits, E=5.5e-11
• PF02719: Polysacc_synt_2
  • amino acids 6 to 118 (113 residues), 20.6 bits, E=4.5e-08

Best Hits

Swiss-Prot: 96% identical to GM4D_SHIFL: GDP-mannose 4,6-dehydratase (gmd) from Shigella flexneri

KEGG orthology group: K01711, GDPmannose 4,6-dehydratase [EC: 4.2.1.47] (inferred from 100% identity to sty:STY2321)

MetaCyc: 96% identical to GDP-mannose 4,6-dehydratase (Escherichia coli K-12 substr. MG1655)
GDP-mannose 4,6-dehydratase. [EC: 4.2.1.47]

Predicted SEED Role

"GDP-mannose 4,6-dehydratase (EC 4.2.1.47)" in subsystem Capsular heptose biosynthesis or Colanic acid biosynthesis (EC 4.2.1.47)

MetaCyc Pathways

• colanic acid building blocks biosynthesis (11/11 steps found)
• GDP-L-fucose biosynthesis I (from GDP-D-mannose) (3/3 steps found)
• GDP-6-deoxy-D-talose biosynthesis (1/2 steps found)
• GDP-D-perosamine biosynthesis (1/2 steps found)
• GDP-D-rhamnose biosynthesis (1/2 steps found)
• GDP-L-colitose biosynthesis (3/5 steps found)
• superpathway of GDP-mannose-derived O-antigen building blocks biosynthesis (9/14 steps found)
• GDP-N-acetyl-α-D-perosamine biosynthesis (1/3 steps found)
• GDP-N-formyl-α-D-perosamine biosynthesis (1/3 steps found)
• GDP-mycosamine biosynthesis (1/3 steps found)
• superpathway of candicidin biosynthesis (4/11 steps found)

KEGG Metabolic Maps

• Fructose and mannose metabolism
• Nucleotide sugars metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.2.1.47

Protein Sequence (373 amino acids)

>GFF3875 GDP-mannose 4,6-dehydratase (EC 4.2.1.47) (Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868)
MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERVDHIYQDPHSCNPKFHL
HYGDLTDASNLTRILQEVQPDEVYNLGAMSHVAVSFESPEYTADVDAMGTLRLLEAIRFL
GLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVAKLYAYWITVNYRESYGIYAC
NGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYLGNMDSLRDWGHAKDYVRMQWMM
LQQEQPEDFVIATGVQYSVRQFVELAAAQLGIKLRFEGEGINEKGIVVSVTGHDAPGVKP
GDVIVAVDPRYFRPAEVETLLGDPSKAHEKLGWKPEITLSEMVSEMVANDLEAAKKHSLL
KSHGYEVAIALES