Protein Info for GFF3866 in Variovorax sp. SCN45

Annotation: HtrA protease/chaperone protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 493 signal peptide" amino acids 1 to 36 (36 residues), see Phobius details TIGR02037: peptidase Do" amino acids 41 to 486 (446 residues), 502.7 bits, see alignment E=4.5e-155 PF13365: Trypsin_2" amino acids 114 to 245 (132 residues), 123.8 bits, see alignment E=2.3e-39 PF00089: Trypsin" amino acids 115 to 265 (151 residues), 61 bits, see alignment E=3.8e-20 PF00595: PDZ" amino acids 280 to 344 (65 residues), 26.9 bits, see alignment E=1.3e-09 PF13180: PDZ_2" amino acids 284 to 375 (92 residues), 55.3 bits, see alignment E=1.7e-18 PF17820: PDZ_6" amino acids 311 to 355 (45 residues), 38.1 bits, see alignment 2.6e-13

Best Hits

Swiss-Prot: 47% identical to DEGPL_PSEF1: Probable periplasmic serine endoprotease DegP-like (Psefu_3239) from Pseudomonas fulva (strain 12-X)

KEGG orthology group: K01362, [EC: 3.4.21.-] (inferred from 94% identity to vpe:Varpa_1539)

Predicted SEED Role

"HtrA protease/chaperone protein"

Isozymes

Compare fitness of predicted isozymes for: 3.4.21.-

Use Curated BLAST to search for 3.4.21.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (493 amino acids)

>GFF3866 HtrA protease/chaperone protein (Variovorax sp. SCN45)
MIFKVEGHKLRSGVLAFALALTAGATFLPVEPVHAQTRTLPDFTDLVDQVGPSVVNIRTV
EKVTPRAGNGEMDEEMQEFFRRFFGQPLPGAPRSGPRPNRPQQPQEEERPRGVGSGFILT
ADGYVMTNAHVVEDASEVLVTLPDKREFKAKIVGADKRSDVAVVKIEATGLPAVKVGDIS
KLRVGEWVMAIGSPFGLENTVTAGIVSAKQRDTGEYLPFIQTDVAINPGNSGGPLINMRG
EVVGINSQIYSRSGGFMGISFSIPIDEAIRVSDQLRTSGRVSRGRIGVQIDQVTKDVAEA
IGLGKAQGALVRGVEAGSPGEKAGVEPGDVITKFDGKAIEKPSDLPRLVGNTKPGTKSTL
TVFRRGALRDLNVTIAEIEPDKPSKRASDRDETPAKPPASAAAKSLGLAVSDLTDVQKKE
LKLKGGVKVDAASDAAARAGLREGDIILAVGNSDISNAREFESAIGKADKSKALSVLFRR
GDWAQYALIRPAR