Protein Info for GFF3859 in Pseudomonas sp. DMC3

Annotation: Acetoin catabolism regulatory protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 636 PF01590: GAF" amino acids 73 to 204 (132 residues), 44.3 bits, see alignment E=8.1e-15 PF00158: Sigma54_activat" amino acids 336 to 498 (163 residues), 216.3 bits, see alignment E=6.5e-68 PF14532: Sigma54_activ_2" amino acids 341 to 503 (163 residues), 63.9 bits, see alignment E=5.7e-21 PF07728: AAA_5" amino acids 355 to 474 (120 residues), 22.4 bits, see alignment E=3.2e-08 PF02954: HTH_8" amino acids 590 to 630 (41 residues), 53.1 bits, see alignment 6.2e-18

Best Hits

KEGG orthology group: None (inferred from 92% identity to pfo:Pfl01_4988)

Predicted SEED Role

"Nitrogen regulation protein NR(I)" in subsystem Ammonia assimilation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (636 amino acids)

>GFF3859 Acetoin catabolism regulatory protein (Pseudomonas sp. DMC3)
MHDNHLSRHARQVLTVTQGKPHLHGPGADPSIARSWLRCLEDYHLDPSVTMAPTVLEHGR
VLESRERLQQVLQIAGTEMSSLHQQLSGAGHAVLLTDARGVILNCVTAPAERKIFERAGL
WLGADWSEACEGTNGIGTCLVERQALTIHQDEHFRGRHTGLTCSASPVFDPHGELLAVLD
VSSARPDVSRQSQFHTMALVNLSAKMIESCYFLRCFDNQWLLRFHLQAESVGLFSEGLLA
FDGEGRISAVNQSALNLLGHIRGGLLGKPVEAFFDCSLDDLLGRASANASASWPLRTRDG
RHLFAVLRGESRKPTAIVPAPIAAPAPRLSGICLGDEALQAEFRKALRVFERDVPLLING
ETGSGKEAFAKAVHQASQRSGNAFVALNCAAIPESLIESELFGYRGGSFTGARKDGMRGK
LQQADGGTLFLDEIGDMPLALQTRLLRVLEDRQVVPIGGEPQAVDVRIISATHRNLLERV
EDGSFREDLYYRLNGLEVALPALRERSDKSQLLDFLLAEEAGGETILIDEPARQALLAFN
WPGNVRQLRNVLRTLAALCDGGRVGVEDLPVMIRQARPAVVAAQIVAQPLDDAERVALLN
ALEQTRWHMTHTAEQLGVSRNTLYRKLRKHGIARSA