Protein Info for GFF385 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: B12 binding domain / kinase domain / Methylmalonyl-CoA mutase (EC 5.4.99.2)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02310: B12-binding
  • amino acids 21 to 124 (104 residues), 52.8 bits, E=6.9e-18
• PF03308: MeaB
  • amino acids 176 to 408 (233 residues), 130.6 bits, E=1.2e-41
• PF02492: cobW
  • amino acids 206 to 379 (174 residues), 23.2 bits, E=9.6e-09
• TIGR00641: methylmalonyl-CoA mutase N-terminal domain
  • amino acids 572 to 1096 (525 residues), 379.7 bits, E=1.8e-117
• PF01642: MM_CoA_mutase
  • amino acids 573 to 1090 (518 residues), 416.8 bits, E=2.5e-128

Best Hits

KEGG orthology group: K11942, methylmalonyl-CoA mutase [EC: 5.4.99.2] (inferred from 78% identity to afw:Anae109_3169)

Predicted SEED Role

"B12 binding domain / kinase domain / Methylmalonyl-CoA mutase (EC 5.4.99.2)" in subsystem Propionyl-CoA to Succinyl-CoA Module or Serine-glyoxylate cycle (EC 5.4.99.2)

MetaCyc Pathways

• propanoyl CoA degradation I (3/3 steps found)
• superpathway of anaerobic energy metabolism (invertebrates) (13/17 steps found)
• 2-oxobutanoate degradation I (3/4 steps found)
• pyruvate fermentation to propanoate I (4/7 steps found)
• conversion of succinate to propanoate (1/3 steps found)
• anaerobic energy metabolism (invertebrates, mitochondrial) (6/10 steps found)
• 3-hydroxypropanoate cycle (8/13 steps found)
• methylaspartate cycle (12/19 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (11/18 steps found)
• (S)-lactate fermentation to propanoate, acetate and hydrogen (7/13 steps found)
• superpathway of L-methionine salvage and degradation (8/16 steps found)
• L-glutamate degradation VIII (to propanoate) (4/11 steps found)
• superpathway of the 3-hydroxypropanoate cycle (9/18 steps found)
• crotonyl-CoA/ethylmalonyl-CoA/hydroxybutyryl-CoA cycle (engineered) (3/14 steps found)

KEGG Metabolic Maps

• Propanoate metabolism
• Valine, leucine and isoleucine degradation

Isozymes

Compare fitness of predicted isozymes for: 5.4.99.2

Use Curated BLAST to search for 5.4.99.2

Protein Sequence (1097 amino acids)

>GFF385 B12 binding domain / kinase domain / Methylmalonyl-CoA mutase (EC 5.4.99.2) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MSNLAAEYQALANYRPTHKVRFVTAASLFDGHDAAINIMRRILQGMGAEVIHLGHNRSVD
EVVTAALQEDAQGIAISSYQGGHVEYFKYMVDLLKSRGGEHIQVFGGGGGVIVPAEIREL
QDYGVTRIYSPEDGQKMGLAGMIGEMVMRCDRDLSPYAPKALAAIEGQGELNWRALAQLI
TVLENGSADAAIVSALREQAKSKKVPVLGITGTGGAGKSSLTDELIRRLRLDQDDRLRVA
VISIDPSRRKSGGALLGDRIRMNAINPWSQGQRVYMRSLATRDFGSEISAALPDVIAACK
CAGFDLIVVETSGIGQGDAAIVPHVDLPMYVMTPEFGAASQLEKIDMLDFAAFVAINKFD
RKGALDALRDVAKQVQRNQEAWSTPPDQMPVFGTMAARFNDDGVTALYQALKVRLGELGL
PLQEGRLPAVKVRHSTNQTPVVPPARTRYLAEISDTVRGYKKKAIEQSRLAREIQQLNAS
AAMLRKDKPERARAAEAVIDLAQLRELDQDPAARKLLAQWPDMQKAYAGDEYVVKIRDKE
IRTALTTKSLSGTVIRKVALPKYEDHGEILKWLMLDNVPGSFPYTAGTFAFKREGEDPTR
MFAGEGDAFRTNRRFKLLSEGMAAKRLSTAFDSVTLYGNDPDPRPDIYGKVGNSGVSIAT
LEDMKVLYGGFDLCHPATSVSMTINGPAPSILAMFMNTAIDQNLEKFETENGRQPTATEA
AKIREWVLANVRGTVQADILKEDQGQNTCIFSTEFSLKVMGDIAEYFVHHDVRNFYSVSI
SGYHIAEAGANPISQLAFTLSNGFTFVEAYLARGMHIDDFAPNLSFFFSNGMDPEYTVLG
RVARRIWATSMRDKYGANERSQKLKYHVQTSGRSLHAQEIQFNDIRTTLQALIAIYDNCN
SLHTNAFDEAITTPTEDSVRRAMAIQLIINREWGLAKNENPNQGAFIIDELTELVEEAVL
QEFEKIAERGGVLGAMETGYQRGKIQDESMHYEMLKHTGEYPIVGVNTFRNPHGDLTPET
LELARSTDDEKQNQLKRLADFHARHADEAPAMLQRLRQAVIENGNVFEVLMDAVRVCSLG
QITNALFEVGGQYRRNM