Protein Info for GFF385 in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868

Annotation: UDP-glucose:(glucosyl)lipopolysaccharide alpha-1,2-glucosyltransferase (EC 2.4.1.58)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 336 PF01501: Glyco_transf_8" amino acids 27 to 274 (248 residues), 246.1 bits, see alignment E=6.5e-77 PF18404: Glyco_transf_24" amino acids 36 to 221 (186 residues), 32.5 bits, see alignment E=9.4e-12 PF08437: Glyco_transf_8C" amino acids 276 to 332 (57 residues), 82.8 bits, see alignment E=2.1e-27

Best Hits

Swiss-Prot: 100% identical to RFAJ_SALTY: Lipopolysaccharide 1,2-glucosyltransferase (rfaJ) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K03279, UDP-glucose:(galactosyl)LPS alpha-1,2-glucosyltransferase [EC: 2.4.1.58] (inferred from 99% identity to sek:SSPA3332)

MetaCyc: 100% identical to lipopolysaccharide glucosyltransferase II (Salmonella enterica enterica serovar Typhimurium str. LT2)
2.4.1.-

Predicted SEED Role

"UDP-glucose:(glucosyl)lipopolysaccharide alpha-1,2-glucosyltransferase (EC 2.4.1.58)" in subsystem LOS core oligosaccharide biosynthesis (EC 2.4.1.58)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.1.58

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (336 amino acids)

>GFF385 UDP-glucose:(glucosyl)lipopolysaccharide alpha-1,2-glucosyltransferase (EC 2.4.1.58) (Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868)
MDSFPEIEIAEYKVFDESNNNDDNVLNISYGVDENYLDGVGVSIASVVLNNNIPLAFHII
CDSYSPCFVKYIERLAVQHHIKISLYLIKVESLEVLPQTKVWSRAMYFRLFAFDYLSKKV
NTLLYLDADVVCKGSLQDLLQLDLTEKIAAVVKDVDSIQNKVNERLSAFNLQGGYFNSGV
VFVNLKLWKENALTKKAFLLLAGKEADSFKYPDQDVLNILLQDKVIFLPRPYNTIYTIKS
ELKDKSHKKYSNIINDNTILIHYTGATKPWHAWANYPSVIYYKNARLNSPWKDFPAKDAR
TIVEFKKRYKHLLVQGHYFKGLLAGSAYLYRKLFHK