Protein Info for PS417_19675 in Pseudomonas simiae WCS417

Annotation: chemotaxis protein CheY

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 375 PF00072: Response_reg" amino acids 5 to 109 (105 residues), 75.7 bits, see alignment E=3.1e-25 PF01339: CheB_methylest" amino acids 194 to 370 (177 residues), 211 bits, see alignment E=1.1e-66

Best Hits

Swiss-Prot: 92% identical to CHEB2_PSEF5: Protein-glutamate methylesterase/protein-glutamine glutaminase 2 (cheB2) from Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)

KEGG orthology group: K03412, two-component system, chemotaxis family, response regulator CheB [EC: 3.1.1.61] (inferred from 93% identity to pfs:PFLU4413)

Predicted SEED Role

"Chemotaxis response regulator protein-glutamate methylesterase CheB (EC 3.1.1.61)" in subsystem Bacterial Chemotaxis (EC 3.1.1.61)

Isozymes

Compare fitness of predicted isozymes for: 3.1.1.61

Use Curated BLAST to search for 3.1.1.61

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See U1UYB7 at UniProt or InterPro

Protein Sequence (375 amino acids)

>PS417_19675 chemotaxis protein CheY (Pseudomonas simiae WCS417)
MAVKVLVVDDSGFFRRRVSEILSADPSIQVVGTATNGKEAIDQAIALKPDVITMDYEMPM
MDGITAVRHIMQRCPTPVLMFSSLTHEGARVTLDALDAGAVDFLPKNFEDISRNPEKVKQ
MLCEKVHSISRSNRRSLFSAPAPAPVAAPAAPTSSSYSRPAPAPVARPAAPPVRTAAPAA
AYSPAPKRKAYKLVAIGTSTGGPVALQRVLTQLPANFPAPIVLIQHMPAAFTKAFAERLD
KLCRISVKEAEDGDILRPGLALLAPGGKQMMVDGRGAIKILPGDERLNYKPCVDITFGSA
AKSYGDKVLAVVLTGMGADGREGARLLKQGGSAIWAQDEASCVIYGMPMAIVKADLADAV
YSLDDIGKHLVEACL