Protein Info for GFF3842 in Variovorax sp. SCN45

Annotation: Uncharacterized protein EC-HemY in Proteobacteria (unrelated to HemY-type PPO in GramPositives)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 427 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details transmembrane" amino acids 43 to 67 (25 residues), see Phobius details TIGR00540: heme biosynthesis-associated TPR protein" amino acids 3 to 420 (418 residues), 257.6 bits, see alignment E=8.1e-81 PF07219: HemY_N" amino acids 26 to 122 (97 residues), 67.9 bits, see alignment E=4e-23

Best Hits

KEGG orthology group: K02498, HemY protein (inferred from 91% identity to vpe:Varpa_1516)

Predicted SEED Role

"Uncharacterized protein EC-HemY, likely associated with heme metabolism based on gene clustering with hemC, hemD in Proteobacteria (unrelated to HemY-type PPO in GramPositives)"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (427 amino acids)

>GFF3842 Uncharacterized protein EC-HemY in Proteobacteria (unrelated to HemY-type PPO in GramPositives) (Variovorax sp. SCN45)
MRAAIWLLALFAVAAAVALFAGNNQGTITVFWPPWRVDLSLNLVLVILLVAFVLLHAALR
ALAALFSLPKQARQWRLQQKERTLHAALLDAMVQLISGRFSRARKAAQAALTQEKTLSAL
GANLPQAQQVRVLSHLLAAESAQALQDKPARDAHLQQALNESADRTVLASPETREGVQLR
AARWALEDRDAAAALARLEELPQGAQRRTLALRLRLKAARQDRRTLEALETARLLAKHRA
FSEAAAQSIVRGLATELLAGAHDPAQLLRAWSELDANERQMPEVAIHAAQRMVALRGDLA
LARGWLLPAWEHMVANPRSLGDALRVKLARALEAGLDSVDAEWLARIESAQRNNPRDPNL
QYLAGMACMKRQLWGKAQQLLMQAGLGLQDTDLYRRAWLALARLAEDRNDTAQAAEAWKR
AAQVESA