Protein Info for GFF3841 in Variovorax sp. SCN45

Annotation: Uncharacterized protein EC-HemX

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF04375: HemX
  • amino acids 133 to 347 (215 residues), 95.9 bits, E=1.6e-31

Best Hits

KEGG orthology group: K02496, uroporphyrin-III C-methyltransferase [EC: 2.1.1.107] (inferred from 86% identity to vpe:Varpa_1515)

Predicted SEED Role

"Homolog of E. coli HemX protein"

MetaCyc Pathways

• siroheme biosynthesis (3/4 steps found)
• adenosylcobalamin biosynthesis II (aerobic) (23/33 steps found)
• factor 430 biosynthesis (3/7 steps found)
• adenosylcobalamin biosynthesis I (anaerobic) (22/36 steps found)
• cob(II)yrinate a,c-diamide biosynthesis II (late cobalt incorporation) (4/13 steps found)
• cob(II)yrinate a,c-diamide biosynthesis I (early cobalt insertion) (5/15 steps found)

KEGG Metabolic Maps

• Porphyrin and chlorophyll metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.107

Use Curated BLAST to search for 2.1.1.107

Protein Sequence (364 amino acids)

>GFF3841 Uncharacterized protein EC-HemX (Variovorax sp. SCN45)
MSAASPSDDLSPPSAATVPVPATVAPHQTPAAQAAGAVILSRLGFGLVAFIALVALIMAI
ALWQKVSGMKEQLARQSADALSQSMEARTLARQASDTVRDASARVALVETRVAEVALQRS
QLEELIQSLSRSRDENLVIDIESAVRLALQQAQVTGSVEPLLAALKAGDVRISRAAQPRL
APLQRAMQRDADRLRSSASTDNAEALQRLDELMRGVDDLPTLNAVALRGTGANAWQAEPI
PADAPWWQRMLLAIRGEARSLVRVGRIQSPEAVLLAPEQSYFLRENLKLKLLNARLSLLS
RQVDATRTELSQVAASLNRYFDPASRRTQAAATLLQQLQQQVRTTEPVRIDDTLAALATA
AAGR