Protein Info for GFF3824 in Variovorax sp. SCN45

Annotation: Argininosuccinate lyase (EC 4.3.2.1)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00838: argininosuccinate lyase
  • amino acids 13 to 464 (452 residues), 608.3 bits, E=5.2e-187
• PF00206: Lyase_1
  • amino acids 17 to 309 (293 residues), 255.1 bits, E=1.1e-79
• PF14698: ASL_C2
  • amino acids 372 to 440 (69 residues), 92.4 bits, E=1.9e-30

Best Hits

Swiss-Prot: 96% identical to ARLY_VARPS: Argininosuccinate lyase (argH) from Variovorax paradoxus (strain S110)

KEGG orthology group: K01755, argininosuccinate lyase [EC: 4.3.2.1] (inferred from 97% identity to vpe:Varpa_1501)

MetaCyc: 51% identical to argininosuccinate lyase (Escherichia coli K-12 substr. MG1655)
Argininosuccinate lyase. [EC: 4.3.2.1]

Predicted SEED Role

"Argininosuccinate lyase (EC 4.3.2.1)" in subsystem Arginine Biosynthesis extended (EC 4.3.2.1)

MetaCyc Pathways

• L-arginine biosynthesis II (acetyl cycle) (10/10 steps found)
• superpathway of arginine and polyamine biosynthesis (15/17 steps found)
• L-arginine biosynthesis I (via L-ornithine) (9/9 steps found)
• L-arginine biosynthesis III (via N-acetyl-L-citrulline) (8/9 steps found)
• urea cycle (4/5 steps found)
• nitric oxide biosynthesis II (mammals) (2/3 steps found)
• superpathway of L-citrulline metabolism (8/12 steps found)
• canavanine biosynthesis (2/4 steps found)
• L-arginine biosynthesis IV (archaea) (4/9 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Arginine and proline metabolism
• Urea cycle and metabolism of amino groups

Isozymes

Compare fitness of predicted isozymes for: 4.3.2.1

Use Curated BLAST to search for 4.3.2.1

Protein Sequence (465 amino acids)

>GFF3824 Argininosuccinate lyase (EC 4.3.2.1) (Variovorax sp. SCN45)
MTQNQFDKKSEAWSALFSEPMSDLVKRYTSSVFFDKRLWQADIQGSLAHAGMLAAQGIIG
VQDHAAIERGMAQIRAEIESGAFEWKLDLEDVHLNIEARLTQLVGDAGKRLHTGRSRNDQ
VATDVRLWLRGEIDLIGDLLVELQKALVDIAEKNVDVILPGFTHLQVAQPVSFGHHMLAY
VEMFSRDAERMGEVRKRVNRLPLGAAALAGTSYPLDRELVAKTLNMDGVCQNSLDAVSDR
DFAIEFTAAASLCMVHVSRMSEELILWMSQNFGFIQIADRFTTGSSIMPQKKNPDVPELA
RGKTGRVVGHLMALITLMKGQPLAYNKDNQEDKEPLFDTVDTLKDTLRIFAEMIGGITVK
PEAMEAAALRGYATATDLADYLVKKGLPFRDAHETVAHAVKAATSHQVDLAELPLAVLQQ
FNPSIEKDVYDVLSLRGSLNARNILGGTAPAQVKAQVARHRARLG