Protein Info for GFF3824 in Pseudomonas sp. DMC3

Annotation: hypothetical protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF10294: Methyltransf_16
  • amino acids 62 to 168 (107 residues), 42.3 bits, E=2.5e-14
• PF13489: Methyltransf_23
  • amino acids 72 to 215 (144 residues), 31.2 bits, E=6.3e-11
• PF05175: MTS
  • amino acids 79 to 149 (71 residues), 33.6 bits, E=1e-11
• PF03602: Cons_hypoth95
  • amino acids 80 to 130 (51 residues), 24 bits, E=9.5e-09
• PF06325: PrmA
  • amino acids 82 to 153 (72 residues), 55.9 bits, E=1.8e-18
• PF13649: Methyltransf_25
  • amino acids 85 to 154 (70 residues), 29.1 bits, E=4.6e-10
• PF08241: Methyltransf_11
  • amino acids 86 to 154 (69 residues), 23.6 bits, E=2.3e-08

Best Hits

KEGG orthology group: None (inferred from 95% identity to pfo:Pfl01_5022)

Predicted SEED Role

"Glutamate synthase [NADPH] large chain (EC 1.4.1.13)" in subsystem Ammonia assimilation or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 1.4.1.13)

MetaCyc Pathways

• L-citrulline biosynthesis (7/8 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
• L-glutamate and L-glutamine biosynthesis (6/7 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• superpathway of L-citrulline metabolism (9/12 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-glutamine degradation II (1/1 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (3/4 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Glutamate metabolism
• Nitrogen metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.4.1.13

Use Curated BLAST to search for 1.4.1.13

Protein Sequence (217 amino acids)

>GFF3824 hypothetical protein (Pseudomonas sp. DMC3)
MTAPLDLQQALGELLGDARLKACALPGTDLQLWLIDGDNMAREFSQEETQRILHEPPYWS
FCWASGLAVARYLAQFPDWVRGKRVLDFGAGSGIAGIAAVKAGALEVVACDLDPLAIAAC
KANAELNDVQMSYSTDFFAEADRFDLILVADVLYDRANLPLLDAFLTRGREALVADSRVR
DFRHPLYERIEMLEAMTLPDLAEPEEFRHVSLYHARR