Protein Info for GFF38 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: 5'-nucleotidase (EC 3.1.3.5)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02872: 5_nucleotid_C
  • amino acids 416 to 573 (158 residues), 100.8 bits, E=1.3e-32
• PF00353: HemolysinCabind
  • amino acids 649 to 682 (34 residues), 37.1 bits, see alignment (E = 3.7e-13)
• PF08548: Peptidase_M10_C
  • amino acids 657 to 713 (57 residues), 22.8 bits, 1.1e-08
• TIGR03661: type I secretion C-terminal target domain (VC_A0849 subclass)
  • amino acids 693 to 762 (70 residues), 18.2 bits, E=2.4e-07

Predicted SEED Role

"5'-nucleotidase (EC 3.1.3.5)" in subsystem Purine conversions (EC 3.1.3.5)

MetaCyc Pathways

• adenosine nucleotides degradation II (5/5 steps found)
• purine nucleotides degradation I (plants) (10/12 steps found)
• adenosine nucleotides degradation I (7/8 steps found)
• guanosine nucleotides degradation II (4/4 steps found)
• purine nucleotides degradation II (aerobic) (9/11 steps found)
• superpathway of guanosine nucleotides degradation (plants) (5/6 steps found)
• guanosine nucleotides degradation I (3/4 steps found)
• guanosine nucleotides degradation III (3/4 steps found)
• inosine 5'-phosphate degradation (3/4 steps found)
• UTP and CTP dephosphorylation I (5/7 steps found)
• ureide biosynthesis (5/7 steps found)
• NAD salvage pathway III (to nicotinamide riboside) (2/3 steps found)
• superpathway of purines degradation in plants (12/18 steps found)
• NAD salvage (plants) (6/11 steps found)
• tunicamycin biosynthesis (1/9 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Nicotinate and nicotinamide metabolism
• Purine metabolism
• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.1.3.5

Use Curated BLAST to search for 3.1.3.5

Protein Sequence (776 amino acids)

>GFF38 5'-nucleotidase (EC 3.1.3.5) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MNASRQLFRRTACAFAASLVLCGGSVWAEGLTVLHLGDQESWLLSAQGNLRDDASQAISF
YGGVDRLATVVANRKAAAQSAGRSVITLNAGDSFLPGPRLTASFANLAGAHADGGQDFYD
AIANRLIGVDATTFGNHEFDLDNTGPVAARFAEVSGTTYLSVNLDFSATPEFANLQAQGK
VAASKILTTAGGKKVGVIGVTTPLLPSISSPPAGIMKNWSAANSELQNLQALLPLVQAEI
DDLRNNQGVTAIVVMSHLQNSQNELQVMVPALSGVDLVISGGGHELMTDPDDLLINGGVA
PTYSTHPVYATDAQNKAVPLVTGHFGNRYVGEVNLTLDDTTGQVTSIDSTRMVRVSGAAA
DADRVTGHAGVAAAVVAPVSSYIAALNAQVIGTTAVPLNGPTHVACPAAPCDYTEGVRNA
DTGLGNLVADAMRFAGKADVGIQNGGGIRTSVAGPGNVTTGDTFNVLPFTNLVKRAPAMN
AAQLKDLLEHSVAASTANGVPQGRFAQVSGIQVVYDTLRTARGTALNSGDRIRRITLDDG
TVLVDNGAVVSPRIFSFATIDFTANGGDGFPFAANAVVFENNVNTITYQEALASFIRTPK
AEGGLQRLNAADGDEITANMYGEENAFDRGGRLIDAAVARTVAGVTRNGTSGRDTLVGTA
GDDLIIGGAGADTLTGGAGLDTFRYTSVRDAGDTITDFTPYVDKLDLNPLLASIGVVGNG
VASGHVSLVDTLGGVHVMLDTDGASGPIAPRPLVTLKGLTAAQFAPGRDLVPAPAP