Protein Info for GFF38 in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868

Annotation: DNA polymerase III alpha subunit (EC 2.7.7.7)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1144 TIGR00594: DNA polymerase III, alpha subunit" amino acids 1 to 1015 (1015 residues), 1439.4 bits, see alignment E=0 PF02811: PHP" amino acids 2 to 156 (155 residues), 122 bits, see alignment E=9.8e-39 PF07733: DNA_pol3_alpha" amino acids 275 to 542 (268 residues), 354.9 bits, see alignment E=9.1e-110 PF17657: DNA_pol3_finger" amino acids 545 to 719 (175 residues), 224.4 bits, see alignment E=1.6e-70 PF14579: HHH_6" amino acids 792 to 882 (91 residues), 96 bits, see alignment E=3.7e-31 PF01336: tRNA_anti-codon" amino acids 984 to 1056 (73 residues), 43.2 bits, see alignment 9.3e-15 PF20914: DNA_pol_IIIA_C" amino acids 1084 to 1135 (52 residues), 98.5 bits, see alignment 4.5e-32

Best Hits

Swiss-Prot: 100% identical to DPO3A_SALTY: DNA polymerase III subunit alpha (dnaE) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K02337, DNA polymerase III subunit alpha [EC: 2.7.7.7] (inferred from 76% identity to avr:B565_3040)

MetaCyc: 97% identical to DNA polymerase III subunit alpha (Escherichia coli K-12 substr. MG1655)
DNA-directed DNA polymerase. [EC: 2.7.7.7]

Predicted SEED Role

"DNA polymerase III alpha subunit (EC 2.7.7.7)" in subsystem DNA-replication (EC 2.7.7.7)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.7

Use Curated BLAST to search for 2.7.7.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1144 amino acids)

>GFF38 DNA polymerase III alpha subunit (EC 2.7.7.7) (Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868)
MIDGLAKTGPLVKKAASLGMPALAITDFTNLCGLVKFYGAGHGAGIKPIVGADFNVHNEL
LGDELTHLTVLAANNTGYQNLTLLISKAYQRGYGAAGPIIERDWLVELKEGLILLSGGRM
GDVGRCLLRGNQALVEECVAFYEAHFPDRYFLELIRTGRQDEETYLHAAVELAEARGLPV
VATNDVRFLESDDFDAHEIRVAIHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFSDIPEA
LENTVEIAKRCNVTVRLGEYFLPQFPTGDMTTEDYLVKKAKEGLEERLAFLFPDEEERKK
RRPEYDERLDIELQVINQMGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKI
TDLDPLEFDLLFERFLNPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTM
AAKAVIRDVGRVLGHPYGFVDRISKLVPPDPGMTLAKAFEAEPQLPEIYEADEEVRALID
MARKLEGVTRNAGKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDF
LGLRTLTIINWALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGM
KDLIKRLQPDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREELSYPDVQWQHESLKPV
LEPTYGIILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFEEGAKKNGI
DGELAMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEK
VVGLVDECWRMGLKILPPDINSGLYHFHVNDEGEIVYGIGAIKGVGEGPIEAIIDARNQG
GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAKAE
AIGQTDMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKEIERY
VGGVRLKDMHPTERGKVTTAAGLVIAARVMVTKRGNRIGICTLDDRSGRLEVMLFTDALD
KYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISLTDRQIDDQ
LLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDLRGLIGSEQVE
LEFD